Detailed information of TA system TA046142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2237189..2237718
• Replicon: chromosome
• Accession: NZ_CP007176
• Organism: Staphylococcus aureus USA300-ISMMS1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AZ30_RS11475
• Protein ID: WP_000621175.1
• Coordinates: 2237189..2237551 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: AZ30_RS11480
• Protein ID: WP_000948331.1
• Coordinates: 2237548..2237718 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZ30_RS11450	2234167..2234937	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
AZ30_RS11455	2234912..2235391	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
AZ30_RS11460	2235393..2235719	-	327	WP_001052491.1	anti-sigma factor antagonist	-
AZ30_RS11465	2235838..2236839	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
AZ30_RS11475	2237189..2237551	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AZ30_RS11480	2237548..2237718	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AZ30_RS11485	2237803..2238951	-	1149	WP_001281145.1	alanine racemase	-
AZ30_RS11490	2239017..2239376	-	360	WP_000581200.1	holo-ACP synthase	-
AZ30_RS11495	2239380..2239871	-	492	WP_001205910.1	PH domain-containing protein	-
AZ30_RS11500	2239858..2241441	-	1584	WP_001294626.1	PH domain-containing protein	-
AZ30_RS11505	2241434..2241913	-	480	WP_001287088.1	hypothetical protein	-
AZ30_RS11510	2242121..2242681	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T46142 WP_000621175.1 NZ_CP007176:c2237551-2237189 [Staphylococcus aureus USA300-ISMMS1]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T46142 NZ_CP007176:c2237551-2237189 [Staphylococcus aureus USA300-ISMMS1]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT46142 WP_000948331.1 NZ_CP007176:c2237718-2237548 [Staphylococcus aureus USA300-ISMMS1]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT46142 NZ_CP007176:c2237718-2237548 [Staphylococcus aureus USA300-ISMMS1]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1