Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1299061..1299977 Replicon chromosome
Accession NZ_CP007173
Organism Bacillus subtilis HJ5

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag AW03_RS06795 Protein ID WP_003244695.1
Coordinates 1299231..1299977 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag AW03_RS06790 Protein ID WP_003232646.1
Coordinates 1299061..1299231 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AW03_RS06760 1295920..1296249 + 330 WP_015483132.1 XkdW family protein -
AW03_RS06765 1296246..1296410 + 165 WP_014479563.1 XkdX family protein -
AW03_RS06770 1296457..1297296 + 840 WP_015383447.1 phage-like element PBSX protein XepA -
AW03_RS06775 1297349..1297618 + 270 WP_015252265.1 hemolysin XhlA family protein -
AW03_RS06780 1297631..1297894 + 264 WP_015483133.1 phage holin -
AW03_RS06785 1297907..1298800 + 894 WP_015483134.1 N-acetylmuramoyl-L-alanine amidase -
AW03_RS21610 1298838..1298975 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
AW03_RS06790 1299061..1299231 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
AW03_RS06795 1299231..1299977 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
AW03_RS06800 1300087..1301088 - 1002 WP_003232642.1 inorganic phosphate transporter -
AW03_RS06805 1301101..1301718 - 618 WP_003218470.1 DUF47 domain-containing protein -
AW03_RS06810 1301994..1303310 - 1317 WP_015383450.1 serine/threonine exchanger -
AW03_RS06815 1303699..1304649 + 951 WP_015383451.1 ring-cleaving dioxygenase -
AW03_RS21615 1304751..1304858 + 108 WP_144052036.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-238)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T46133 WP_003244695.1 NZ_CP007173:c1299977-1299231 [Bacillus subtilis HJ5]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T46133 NZ_CP007173:c1299977-1299231 [Bacillus subtilis HJ5]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTATATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGTGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGACGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT46133 WP_003232646.1 NZ_CP007173:c1299231-1299061 [Bacillus subtilis HJ5]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT46133 NZ_CP007173:c1299231-1299061 [Bacillus subtilis HJ5]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 3O6Q


Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References