Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 493702..494338 Replicon chromosome
Accession NZ_CP007173
Organism Bacillus subtilis HJ5

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag AW03_RS02500 Protein ID WP_003156187.1
Coordinates 493988..494338 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag AW03_RS02495 Protein ID WP_003225183.1
Coordinates 493702..493983 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AW03_RS02475 490061..490660 - 600 WP_015482831.1 rhomboid family intramembrane serine protease -
AW03_RS02480 490755..491120 + 366 WP_015252768.1 holo-ACP synthase -
AW03_RS02485 491286..492302 + 1017 WP_080030712.1 outer membrane lipoprotein carrier protein LolA -
AW03_RS02490 492417..493586 + 1170 WP_015382833.1 alanine racemase -
AW03_RS02495 493702..493983 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
AW03_RS02500 493988..494338 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
AW03_RS02505 494454..495278 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
AW03_RS02510 495283..495648 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
AW03_RS02515 495652..496053 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
AW03_RS02520 496065..497072 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
AW03_RS02525 497134..497463 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
AW03_RS02530 497460..497942 + 483 WP_003234299.1 anti-sigma B factor RsbW -
AW03_RS02535 497908..498696 + 789 WP_015482833.1 RNA polymerase sigma factor SigB -
AW03_RS02540 498696..499295 + 600 WP_015382836.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T46132 WP_003156187.1 NZ_CP007173:493988-494338 [Bacillus subtilis HJ5]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T46132 NZ_CP007173:493988-494338 [Bacillus subtilis HJ5]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT46132 WP_003225183.1 NZ_CP007173:493702-493983 [Bacillus subtilis HJ5]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT46132 NZ_CP007173:493702-493983 [Bacillus subtilis HJ5]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References