Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 503145..503782 Replicon chromosome
Accession NZ_CP007165
Organism Bacillus velezensis NJN-6

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag AW02_RS02370 Protein ID WP_003156187.1
Coordinates 503432..503782 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag AW02_RS02365 Protein ID WP_003156188.1
Coordinates 503145..503426 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AW02_RS02345 499510..500109 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
AW02_RS02350 500202..500567 + 366 WP_003156192.1 holo-ACP synthase -
AW02_RS02355 500732..501739 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
AW02_RS02360 501856..503025 + 1170 WP_003156189.1 alanine racemase -
AW02_RS02365 503145..503426 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
AW02_RS02370 503432..503782 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
AW02_RS02375 503900..504721 + 822 WP_014304404.1 STAS domain-containing protein -
AW02_RS02380 504726..505091 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
AW02_RS02385 505094..505495 + 402 WP_003156178.1 anti-sigma regulatory factor -
AW02_RS02390 505507..506514 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
AW02_RS02395 506578..506907 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
AW02_RS02400 506904..507386 + 483 WP_003156173.1 anti-sigma B factor RsbW -
AW02_RS02405 507352..508140 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
AW02_RS02410 508140..508742 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T46125 WP_003156187.1 NZ_CP007165:503432-503782 [Bacillus velezensis NJN-6]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T46125 NZ_CP007165:503432-503782 [Bacillus velezensis NJN-6]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT46125 WP_003156188.1 NZ_CP007165:503145-503426 [Bacillus velezensis NJN-6]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT46125 NZ_CP007165:503145-503426 [Bacillus velezensis NJN-6]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References