Detailed information of TA system TA046124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 547614..548262
• Replicon: chromosome
• Accession: NZ_CP007161
• Organism: Virgibacillus sp. SK37

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A6L8JWB3
• Locus tag: X953_RS02835
• Protein ID: WP_019379217.1
• Coordinates: 547903..548262 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: X953_RS02830
• Protein ID: WP_084715600.1
• Coordinates: 547614..547898 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
X953_RS02810	543097..543456	+	360	WP_040954276.1	holo-ACP synthase	-
X953_RS02815	543516..545036	+	1521	WP_040954277.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
X953_RS02820	545126..546136	+	1011	WP_040954278.1	outer membrane lipoprotein carrier protein LolA	-
X953_RS02825	546327..547451	+	1125	WP_040954279.1	alanine racemase	-
X953_RS02830	547614..547898	+	285	WP_084715600.1	antitoxin	Antitoxin
X953_RS02835	547903..548262	+	360	WP_019379217.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
X953_RS02840	549235..550105	+	871	Protein_546	RsbT co-antagonist protein RsbRA	-
X953_RS02845	550111..550467	+	357	WP_040954281.1	STAS domain-containing protein	-
X953_RS02850	550470..550871	+	402	WP_040954282.1	anti-sigma regulatory factor	-
X953_RS02855	550987..552000	+	1014	WP_040954283.1	PP2C family protein-serine/threonine phosphatase	-
X953_RS02860	552105..552437	+	333	WP_040954284.1	STAS domain-containing protein	-
X953_RS02865	552437..552913	+	477	WP_040954285.1	anti-sigma B factor RsbW	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13377.53 Da
• Isoelectric Point: 8.4940

>T46124 WP_019379217.1 NZ_CP007161:547903-548262 [Virgibacillus sp. SK37]
MIVQRGEVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKRYGFDRNSVILLEQI
RTLDKQRLTDKITKLDAEMMNKINQALEISLGLKDMYDH

Nucleotide

• Length: 360 bp

>T46124 NZ_CP007161:547903-548262 [Virgibacillus sp. SK37]
TTGATCGTTCAAAGAGGTGAAGTATATTTCGCCGACTTATCCCCTGTGGTTGGATCGGAGCAGGGAGGCGTGCGCCCGGT
ATTGATCCTTCAAAATGATATAGGTAATCGATTTAGCCCGACCGTTATTGTGGCAGCTATTACTGCGCAAATTCAAAAGG
CTAAGTTACCTACTCATGTTGAAATTAATGCAAAACGCTACGGATTCGACCGAAACTCTGTTATTCTTCTGGAACAGATC
CGTACATTGGATAAACAAAGATTAACAGATAAAATAACTAAACTTGACGCTGAAATGATGAATAAAATAAATCAGGCTCT
GGAAATTAGTCTGGGGCTAAAAGATATGTATGACCATTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10924.27 Da
• Isoelectric Point: 4.3837

>AT46124 WP_084715600.1 NZ_CP007161:547614-547898 [Virgibacillus sp. SK37]
MSESLQEILVKIPKNLLNEVDGLMRYENSDLSDFICQATRSYLQHKKEEHIQQFRETMQKGYEEMGRINLTIASEAFQAE
EEAENTLERSVIGV

Nucleotide

• Length: 285 bp

>AT46124 NZ_CP007161:547614-547898 [Virgibacillus sp. SK37]
TTGTCAGAGAGCTTACAGGAGATCTTAGTGAAAATACCAAAAAACCTATTGAATGAGGTGGATGGATTAATGAGATATGA
AAATAGTGACTTAAGTGACTTCATTTGTCAGGCTACAAGAAGCTACTTGCAGCATAAAAAAGAGGAACATATCCAACAAT
TCCGTGAGACAATGCAAAAAGGCTATGAAGAAATGGGTCGCATTAATCTAACAATTGCTTCGGAAGCTTTTCAAGCTGAA
GAGGAGGCTGAAAACACCTTAGAGCGCTCTGTGATCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A6L8JWB3