Detailed information of TA system TA046123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 8543215..8543843
• Replicon: chromosome
• Accession: NZ_CP007155
• Organism: Kutzneria albida DSM 43870

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KALB_RS37170
• Protein ID: WP_025360753.1
• Coordinates: 8543215..8543613 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: W5WJ68
• Locus tag: KALB_RS37175
• Protein ID: WP_025360754.1
• Coordinates: 8543610..8543843 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KALB_RS37135	8539355..8540116	-	762	WP_201771861.1	alpha/beta hydrolase	-
KALB_RS37140	8540185..8540754	+	570	WP_035968150.1	AAA family ATPase	-
KALB_RS37145	8540764..8541288	+	525	WP_025360748.1	GNAT family N-acetyltransferase	-
KALB_RS37150	8541298..8541816	+	519	WP_025360749.1	GNAT family N-acetyltransferase	-
KALB_RS37155	8541783..8542520	-	738	WP_025360750.1	alpha/beta hydrolase	-
KALB_RS46035	8542625..8542906	-	282	WP_148310007.1	hypothetical protein	-
KALB_RS37165	8543012..8543218	+	207	WP_025360752.1	zinc ribbon domain-containing protein	-
KALB_RS37170	8543215..8543613	-	399	WP_025360753.1	type II toxin-antitoxin system VapC family toxin	Toxin
KALB_RS37175	8543610..8543843	-	234	WP_025360754.1	antitoxin	Antitoxin
KALB_RS37180	8543890..8545671	-	1782	WP_025360755.1	NAD(P)-binding domain-containing protein	-
KALB_RS37185	8545825..8546367	-	543	WP_042223062.1	class F sortase	-
KALB_RS37190	8546517..8547080	-	564	WP_025360757.1	hypothetical protein	-
KALB_RS37195	8547162..8547833	-	672	WP_025360758.1	PTS-dependent dihydroxyacetone kinase phosphotransferase subunit DhaM	-
KALB_RS37200	8547830..8548459	-	630	WP_025360759.1	dihydroxyacetone kinase subunit L	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14235.43 Da
• Isoelectric Point: 8.4925

>T46123 WP_025360753.1 NZ_CP007155:c8543613-8543215 [Kutzneria albida DSM 43870]
VNDLVVDASVYIYASTSTEPRARQLLDRIRTATCHAPHLFDAEVGNVLRRKELAGQITANVALVSLRAVKHLISERYPAV
GPLAEAAWRLRGAVTFYDALYVVLAASLGSPLVTGDARLSRAPGLPCQVELV

Nucleotide

• Length: 399 bp

>T46123 NZ_CP007155:c8543613-8543215 [Kutzneria albida DSM 43870]
GTGAACGACCTGGTCGTCGACGCGTCCGTGTACATCTACGCCTCGACCTCGACCGAGCCGCGTGCTCGGCAGTTGCTCGA
CCGGATCCGGACCGCCACCTGTCACGCCCCGCACCTGTTCGACGCCGAGGTGGGCAACGTACTCAGGCGCAAGGAACTGG
CCGGGCAGATCACCGCGAACGTGGCCCTGGTCTCGCTGCGCGCGGTCAAGCACCTGATCTCCGAGCGGTACCCGGCGGTG
GGTCCGCTGGCGGAGGCGGCCTGGCGACTGCGCGGGGCGGTCACGTTCTACGACGCGCTCTACGTGGTACTCGCGGCCTC
ACTCGGCTCCCCGCTGGTCACCGGCGACGCCCGGTTGAGCCGGGCACCCGGACTGCCCTGCCAGGTCGAACTGGTCTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8520.70 Da
• Isoelectric Point: 4.7085

>AT46123 WP_025360754.1 NZ_CP007155:c8543843-8543610 [Kutzneria albida DSM 43870]
VATIQVRDIPEDAYETLRKRARAAGQSIQAYMRDQIIALASQRTKEEVVAAIDMTLLNEGTAITMDSIVTDLAADRR

Nucleotide

• Length: 234 bp

>AT46123 NZ_CP007155:c8543843-8543610 [Kutzneria albida DSM 43870]
GTGGCGACCATCCAGGTTCGGGACATCCCGGAAGACGCGTACGAGACGCTCCGCAAGCGCGCCCGCGCCGCCGGGCAGTC
CATCCAGGCGTACATGCGCGACCAGATCATCGCGCTGGCCTCGCAGCGCACCAAGGAGGAGGTGGTGGCGGCGATCGACA
TGACCCTGCTGAACGAGGGCACGGCGATCACCATGGACAGCATCGTCACCGACCTGGCGGCTGACCGCAGGTGA

Structures

Antitoxin

• AlphaFold DB: W5WJ68