Detailed information of TA system TA046112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 269694..270274
• Replicon: plasmid pHsw1
• Accession: NZ_CP007144
• Organism: Hymenobacter swuensis DY53

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: W8EZ00
• Locus tag: HSW_RS01370
• Protein ID: WP_044000468.1
• Coordinates: 269694..270026 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: W8EV10
• Locus tag: HSW_RS01375
• Protein ID: WP_044000469.1
• Coordinates: 270014..270274 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HSW_RS01345	264848..266197	+	1350	WP_044000463.1	hypothetical protein	-
HSW_RS01350	266265..267137	-	873	WP_044000464.1	DUF2971 domain-containing protein	-
HSW_RS01355	267340..267840	+	501	WP_044000465.1	SMI1/KNR4 family protein	-
HSW_RS01360	268342..268968	+	627	WP_155832769.1	hypothetical protein	-
HSW_RS24000	269090..269248	+	159	WP_155832770.1	hypothetical protein	-
HSW_RS01365	269309..269557	+	249	WP_044000467.1	hypothetical protein	-
HSW_RS01370	269694..270026	-	333	WP_044000468.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HSW_RS01375	270014..270274	-	261	WP_044000469.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HSW_RS01380	270492..270863	-	372	WP_052345982.1	hypothetical protein	-
HSW_RS01385	271441..271797	+	357	WP_155832771.1	hypothetical protein	-
HSW_RS24005	272433..272600	+	168	WP_155832772.1	hypothetical protein	-
HSW_RS23365	273164..273421	+	258	WP_071883036.1	helix-turn-helix domain-containing protein	-
HSW_RS23370	273525..274433	-	909	WP_071883037.1	TniB family NTP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..299298	299298

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12583.47 Da
• Isoelectric Point: 8.7371

>T46112 WP_044000468.1 NZ_CP007144:c270026-269694 [Hymenobacter swuensis DY53]
MAVVAVQRFEVWLINLDPTQGSEINKTRPCVVLSPDEMNRYLRTVTIAALTSTRRDYPSRVDCTFQGKEGQVALDHIRSV
DKTRLVRRLGVLSKATAQEVCDRLQEIFQY

Nucleotide

• Length: 333 bp

>T46112 NZ_CP007144:c270026-269694 [Hymenobacter swuensis DY53]
ATGGCAGTGGTAGCCGTGCAGCGCTTCGAGGTGTGGCTCATTAACCTCGACCCGACCCAGGGCAGCGAAATCAACAAGAC
CCGACCCTGTGTGGTGCTCTCCCCCGACGAGATGAACCGCTACCTGCGCACGGTCACCATCGCGGCTCTGACCAGCACCC
GGCGCGACTACCCGTCCCGGGTAGACTGCACCTTCCAGGGCAAAGAGGGCCAGGTCGCGCTCGACCACATCCGTTCCGTG
GACAAAACCCGTTTGGTGCGCCGCTTAGGCGTATTGTCCAAAGCCACTGCCCAGGAGGTATGTGACCGGCTGCAGGAGAT
ATTTCAGTACTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9904.21 Da
• Isoelectric Point: 4.4750

>AT46112 WP_044000469.1 NZ_CP007144:c270274-270014 [Hymenobacter swuensis DY53]
MNTRLIRVGNSQGIVLPKKLLEQYHLTGEVDLQPTPEGLLIKPVMKPRRQGWDERFQQAIAQGQAPEGELLEGFSDEAFE
ETEWQW

Nucleotide

• Length: 261 bp

>AT46112 NZ_CP007144:c270274-270014 [Hymenobacter swuensis DY53]
ATGAATACGCGCCTGATTCGGGTGGGCAATTCCCAAGGAATTGTGCTGCCCAAGAAGTTACTGGAACAATATCACCTCAC
CGGCGAAGTGGACCTGCAGCCCACGCCTGAAGGCCTGCTCATCAAGCCGGTGATGAAGCCCCGCCGCCAAGGTTGGGACG
AGCGCTTCCAGCAGGCCATTGCCCAGGGCCAGGCCCCGGAGGGCGAGCTGCTGGAGGGATTTTCGGACGAGGCCTTTGAG
GAAACGGAATGGCAGTGGTAG

Structures

Toxin

• AlphaFold DB: W8EZ00

Antitoxin

• AlphaFold DB: W8EV10