Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4334334..4334965 Replicon chromosome
Accession NZ_CP007136
Organism Escherichia coli O145:H28 str. RM12581

Toxin (Protein)


Gene name hicA Uniprot ID A0A7U9LJQ2
Locus tag ECRM12581_RS22285 Protein ID WP_001259387.1
Coordinates 4334690..4334965 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag ECRM12581_RS22280 Protein ID WP_000593555.1
Coordinates 4334334..4334693 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM12581_RS22250 4329446..4329919 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
ECRM12581_RS22255 4329916..4330197 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
ECRM12581_RS22260 4330274..4331632 + 1359 WP_001308116.1 PTS galactitol transporter subunit IIC -
ECRM12581_RS22265 4331625..4333133 + 1509 WP_001400448.1 carbohydrate kinase -
ECRM12581_RS22270 4333123..4333392 + 270 WP_000933061.1 HPr family phosphocarrier protein -
ECRM12581_RS22275 4333424..4334284 + 861 WP_001131788.1 class II aldolase -
ECRM12581_RS22280 4334334..4334693 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ECRM12581_RS22285 4334690..4334965 - 276 WP_001259387.1 type II toxin-antitoxin system HicA family toxin Toxin
ECRM12581_RS22290 4335038..4336162 - 1125 WP_001314210.1 ABC transporter permease -
ECRM12581_RS22295 4336162..4338897 - 2736 WP_000149086.1 ribosome-associated ATPase/putative transporter RbbA -
ECRM12581_RS22300 4338894..4339961 - 1068 WP_000361517.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10214.91 Da        Isoelectric Point: 10.7076

>T46096 WP_001259387.1 NZ_CP007136:c4334965-4334690 [Escherichia coli O145:H28 str. RM12581]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESQVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T46096 NZ_CP007136:c4334965-4334690 [Escherichia coli O145:H28 str. RM12581]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACAGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT46096 WP_000593555.1 NZ_CP007136:c4334693-4334334 [Escherichia coli O145:H28 str. RM12581]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT46096 NZ_CP007136:c4334693-4334334 [Escherichia coli O145:H28 str. RM12581]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAGCTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LJQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References