Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4244628..4245382 Replicon chromosome
Accession NZ_CP007136
Organism Escherichia coli O145:H28 str. RM12581

Toxin (Protein)


Gene name ataT Uniprot ID A0A7U9LH62
Locus tag ECRM12581_RS21820 Protein ID WP_001400419.1
Coordinates 4244897..4245382 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID Q8X700
Locus tag ECRM12581_RS21815 Protein ID WP_000801912.1
Coordinates 4244628..4244906 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM12581_RS21810 4241856..4244561 + 2706 WP_000906970.1 HTH-type transcriptional regulator MalT -
ECRM12581_RS21815 4244628..4244906 + 279 WP_000801912.1 DUF1778 domain-containing protein Antitoxin
ECRM12581_RS21820 4244897..4245382 + 486 WP_001400419.1 GNAT family N-acetyltransferase Toxin
ECRM12581_RS21825 4245432..4246460 - 1029 WP_024221621.1 RNA 3'-terminal phosphate cyclase -
ECRM12581_RS21830 4246464..4247690 - 1227 WP_001105483.1 RNA-splicing ligase RtcB -
ECRM12581_RS21835 4247879..4249477 + 1599 WP_001232936.1 DNA-binding transcriptional regulator RtcR -
ECRM12581_RS21840 4249459..4250217 - 759 WP_000815099.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(49-143)

Antitoxin

Vibrio phage ICP1, Orf50

(2-92)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17692.65 Da        Isoelectric Point: 9.4066

>T46094 WP_001400419.1 NZ_CP007136:4244897-4245382 [Escherichia coli O145:H28 str. RM12581]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSTGLSRVYVICIVNTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Download         Length: 486 bp

>T46094 NZ_CP007136:4244897-4245382 [Escherichia coli O145:H28 str. RM12581]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCACCGGTCTGTCTCGTGTTTACGTTATCTGTATTGTCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10420.94 Da        Isoelectric Point: 4.6336

>AT46094 WP_000801912.1 NZ_CP007136:4244628-4244906 [Escherichia coli O145:H28 str. RM12581]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Download         Length: 279 bp

>AT46094 NZ_CP007136:4244628-4244906 [Escherichia coli O145:H28 str. RM12581]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LH62

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LHB9

References