Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3862865..3863702 Replicon chromosome
Accession NZ_CP007136
Organism Escherichia coli O145:H28 str. RM12581

Toxin (Protein)


Gene name yeeV Uniprot ID A0A7U9LL67
Locus tag ECRM12581_RS19810 Protein ID WP_000854687.1
Coordinates 3862865..3863245 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7U9LJJ3
Locus tag ECRM12581_RS19815 Protein ID WP_001443392.1
Coordinates 3863334..3863702 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM12581_RS19780 3858507..3860348 + 1842 WP_000437371.1 RNA polymerase sigma factor RpoD -
ECRM12581_RS19785 3860427..3860933 - 507 WP_000228947.1 G/U mismatch-specific DNA glycosylase -
ECRM12581_RS19795 3861233..3862078 - 846 WP_001274557.1 DUF4942 domain-containing protein -
ECRM12581_RS19800 3862163..3862360 - 198 WP_000772026.1 DUF957 domain-containing protein -
ECRM12581_RS19805 3862380..3862868 - 489 WP_001054228.1 hypothetical protein -
ECRM12581_RS19810 3862865..3863245 - 381 WP_000854687.1 TA system toxin CbtA family protein Toxin
ECRM12581_RS19815 3863334..3863702 - 369 WP_001443392.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ECRM12581_RS19820 3863778..3863999 - 222 WP_000692312.1 DUF987 domain-containing protein -
ECRM12581_RS19825 3864062..3864538 - 477 WP_001186728.1 RadC family protein -
ECRM12581_RS19830 3864554..3865027 - 474 WP_001444656.1 antirestriction protein -
ECRM12581_RS19835 3865121..3865366 - 246 WP_000680587.1 hypothetical protein -
ECRM12581_RS19840 3865366..3865806 - 441 Protein_3755 DUF932 domain-containing protein -
ECRM12581_RS19845 3865925..3867703 - 1779 WP_001243195.1 reverse transcriptase/maturase family protein -
ECRM12581_RS19850 3868213..3868596 - 384 Protein_3757 DUF932 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 14199.13 Da        Isoelectric Point: 6.8615

>T46092 WP_000854687.1 NZ_CP007136:c3863245-3862865 [Escherichia coli O145:H28 str. RM12581]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNNITLGRHPEEAKQ

Download         Length: 381 bp

>T46092 NZ_CP007136:c3863245-3862865 [Escherichia coli O145:H28 str. RM12581]
ATGAAAACATTACCCGACACTCACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCTCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCCCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTAGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13781.54 Da        Isoelectric Point: 6.2049

>AT46092 WP_001443392.1 NZ_CP007136:c3863702-3863334 [Escherichia coli O145:H28 str. RM12581]
VSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLDDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVALYAKGLTCDADTLGSCGYVYLAVYPTPDMKK

Download         Length: 369 bp

>AT46092 NZ_CP007136:c3863702-3863334 [Escherichia coli O145:H28 str. RM12581]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGACGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTAGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGGCACTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGATATGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LL67

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LJJ3

References