Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3533247..3533830 Replicon chromosome
Accession NZ_CP007136
Organism Escherichia coli O145:H28 str. RM12581

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U9LIA0
Locus tag ECRM12581_RS18190 Protein ID WP_000254747.1
Coordinates 3533247..3533582 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag ECRM12581_RS18195 Protein ID WP_000581937.1
Coordinates 3533582..3533830 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM12581_RS18175 3529134..3530432 - 1299 WP_000036723.1 phosphopyruvate hydratase -
ECRM12581_RS18180 3530520..3532157 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
ECRM12581_RS18185 3532385..3533176 - 792 WP_001071643.1 nucleoside triphosphate pyrophosphohydrolase -
ECRM12581_RS18190 3533247..3533582 - 336 WP_000254747.1 endoribonuclease MazF Toxin
ECRM12581_RS18195 3533582..3533830 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
ECRM12581_RS18200 3533908..3536142 - 2235 WP_000226815.1 GTP pyrophosphokinase -
ECRM12581_RS18205 3536190..3537491 - 1302 WP_000046821.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T46089 WP_000254747.1 NZ_CP007136:c3533582-3533247 [Escherichia coli O145:H28 str. RM12581]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLYVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T46089 NZ_CP007136:c3533582-3533247 [Escherichia coli O145:H28 str. RM12581]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTATGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT46089 WP_000581937.1 NZ_CP007136:c3533830-3533582 [Escherichia coli O145:H28 str. RM12581]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT46089 NZ_CP007136:c3533830-3533582 [Escherichia coli O145:H28 str. RM12581]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LIA0

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References