Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1921829..1922467 Replicon chromosome
Accession NZ_CP007136
Organism Escherichia coli O145:H28 str. RM12581

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ECRM12581_RS09825 Protein ID WP_000813794.1
Coordinates 1922291..1922467 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ECRM12581_RS09820 Protein ID WP_001270286.1
Coordinates 1921829..1922245 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM12581_RS09800 1916980..1917921 - 942 WP_001251328.1 ABC transporter permease -
ECRM12581_RS09805 1917922..1918935 - 1014 WP_000220414.1 ABC transporter ATP-binding protein -
ECRM12581_RS09810 1918953..1920098 - 1146 WP_000335342.1 ABC transporter substrate-binding protein -
ECRM12581_RS09815 1920344..1921750 - 1407 WP_000760665.1 PLP-dependent aminotransferase family protein -
ECRM12581_RS09820 1921829..1922245 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ECRM12581_RS09825 1922291..1922467 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ECRM12581_RS09830 1922689..1922919 + 231 WP_000494244.1 YncJ family protein -
ECRM12581_RS09835 1923011..1924972 - 1962 WP_000218823.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ECRM12581_RS09840 1925045..1925581 - 537 WP_000429147.1 DNA-binding transcriptional regulator SutR -
ECRM12581_RS09845 1925634..1926845 + 1212 WP_071792349.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T46078 WP_000813794.1 NZ_CP007136:c1922467-1922291 [Escherichia coli O145:H28 str. RM12581]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T46078 NZ_CP007136:c1922467-1922291 [Escherichia coli O145:H28 str. RM12581]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT46078 WP_001270286.1 NZ_CP007136:c1922245-1921829 [Escherichia coli O145:H28 str. RM12581]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT46078 NZ_CP007136:c1922245-1921829 [Escherichia coli O145:H28 str. RM12581]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCATTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References