Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1320426..1321221 Replicon chromosome
Accession NZ_CP007136
Organism Escherichia coli O145:H28 str. RM12581

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag ECRM12581_RS06555 Protein ID WP_000854914.1
Coordinates 1320847..1321221 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7U9LM11
Locus tag ECRM12581_RS06550 Protein ID WP_001280953.1
Coordinates 1320426..1320800 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM12581_RS06510 1315781..1316686 + 906 WP_000203541.1 chemotaxis protein -
ECRM12581_RS06515 1316683..1317753 + 1071 WP_000102643.1 patatin-like phospholipase family protein -
ECRM12581_RS06525 1318093..1318911 + 819 WP_001234682.1 DUF945 domain-containing protein -
ECRM12581_RS06530 1319002..1319487 + 486 WP_000214398.1 antirestriction protein -
ECRM12581_RS06535 1319503..1319979 + 477 WP_001186738.1 RadC family protein -
ECRM12581_RS06540 1320042..1320263 + 222 WP_000692323.1 DUF987 domain-containing protein -
ECRM12581_RS06550 1320426..1320800 + 375 WP_001280953.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ECRM12581_RS06555 1320847..1321221 + 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
ECRM12581_RS06560 1321218..1321709 + 492 WP_000976857.1 hypothetical protein -
ECRM12581_RS06565 1321721..1321918 + 198 WP_000839282.1 DUF957 domain-containing protein -
ECRM12581_RS06570 1322003..1322845 + 843 WP_001280481.1 DUF4942 domain-containing protein -
ECRM12581_RS06580 1323316..1324254 + 939 WP_000351301.1 glyoxylate/hydroxypyruvate reductase GhrA -
ECRM12581_RS06585 1324309..1325046 + 738 WP_000283664.1 zinc-binding phosphatase -
ECRM12581_RS06590 1325070..1325624 + 555 WP_001001917.1 molecular chaperone YcdY -
ECRM12581_RS06595 1325726..1326217 + 492 WP_001297187.1 DUF1097 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - csgG / csgF / csgE / csgD / csgB / csgA / csgC 1319503..1331154 11651


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T46073 WP_000854914.1 NZ_CP007136:1320847-1321221 [Escherichia coli O145:H28 str. RM12581]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T46073 NZ_CP007136:1320847-1321221 [Escherichia coli O145:H28 str. RM12581]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13734.56 Da        Isoelectric Point: 6.6249

>AT46073 WP_001280953.1 NZ_CP007136:1320426-1320800 [Escherichia coli O145:H28 str. RM12581]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPALATTS

Download         Length: 375 bp

>AT46073 NZ_CP007136:1320426-1320800 [Escherichia coli O145:H28 str. RM12581]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACTCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LM11

References