Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 54755..55305 Replicon chromosome
Accession NZ_CP007136
Organism Escherichia coli O145:H28 str. RM12581

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag ECRM12581_RS00275 Protein ID WP_001160974.1
Coordinates 54991..55305 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag ECRM12581_RS00270 Protein ID WP_000125566.1
Coordinates 54755..54988 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM12581_RS00245 49826..50113 + 288 WP_000203748.1 ferredoxin-like protein FixX -
ECRM12581_RS00250 50171..51502 + 1332 WP_001183192.1 MFS transporter -
ECRM12581_RS00255 51613..52143 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
ECRM12581_RS00260 52136..53998 + 1863 WP_000377137.1 glutathione-regulated potassium-efflux system protein KefC -
ECRM12581_RS00265 54190..54669 + 480 WP_024221573.1 type 3 dihydrofolate reductase -
ECRM12581_RS00270 54755..54988 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
ECRM12581_RS00275 54991..55305 + 315 WP_001160974.1 CcdB family protein Toxin
ECRM12581_RS00280 55302..56150 - 849 WP_000257203.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
ECRM12581_RS00285 56157..56534 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
ECRM12581_RS00290 56537..57358 - 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
ECRM12581_RS00295 57355..58344 - 990 WP_000241240.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
ECRM12581_RS00300 58344..59630 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T46071 WP_001160974.1 NZ_CP007136:54991-55305 [Escherichia coli O145:H28 str. RM12581]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T46071 NZ_CP007136:54991-55305 [Escherichia coli O145:H28 str. RM12581]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT46071 WP_000125566.1 NZ_CP007136:54755-54988 [Escherichia coli O145:H28 str. RM12581]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT46071 NZ_CP007136:54755-54988 [Escherichia coli O145:H28 str. RM12581]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References