Detailed information of TA system TA046070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 78053..78588
• Replicon: plasmid pO145-12761
• Accession: NZ_CP007135
• Organism: Escherichia coli O145:H28 str. RM12761

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ECRM12761_RS28300
• Protein ID: WP_001270421.1
• Coordinates: 78053..78340 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1V0M724
• Locus tag: ECRM12761_RS28305
• Protein ID: WP_001132893.1
• Coordinates: 78337..78588 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM12761_RS28280	74195..75172	-	978	WP_000361617.1	RepB family plasmid replication initiator protein	-
ECRM12761_RS32735	75818..76106	-	289	Protein_93	integrase core domain-containing protein	-
ECRM12761_RS28290	76291..77478	-	1188	WP_025380712.1	IS91 family transposase	-
ECRM12761_RS28295	77478..77843	-	366	WP_000091308.1	hypothetical protein	-
ECRM12761_RS33075	77929..78051	-	123	Protein_96	IS3 family transposase	-
ECRM12761_RS28300	78053..78340	-	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECRM12761_RS28305	78337..78588	-	252	WP_001132893.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ECRM12761_RS32830	78762..79079	-	318	WP_157910106.1	hypothetical protein	-
ECRM12761_RS28315	79306..80511	+	1206	WP_000817642.1	AAA family ATPase	-
ECRM12761_RS28320	80508..81470	+	963	WP_000756329.1	ParB/RepB/Spo0J family partition protein	-
ECRM12761_RS28325	81610..81978	-	369	Protein_102	DUF4113 domain-containing protein	-
ECRM12761_RS28330	82166..83353	-	1188	WP_025380712.1	IS91 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	stcE / exeE / exeG / espP / hlyC / hlyA / hlyB / hlyD	1..98067	98067
	-	inside	IScluster/Tn	-	-	68967..84326	15359

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T46070 WP_001270421.1 NZ_CP007135:c78340-78053 [Escherichia coli O145:H28 str. RM12761]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T46070 NZ_CP007135:c78340-78053 [Escherichia coli O145:H28 str. RM12761]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAG
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9192.50 Da
• Isoelectric Point: 4.2108

>AT46070 WP_001132893.1 NZ_CP007135:c78588-78337 [Escherichia coli O145:H28 str. RM12761]
MPNIILSDTSASVSELKKNPMATVSAGDGFPIAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT46070 NZ_CP007135:c78588-78337 [Escherichia coli O145:H28 str. RM12761]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGATCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTAGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A1V0M724