Detailed information of TA system TA046065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 8472..8973
• Replicon: plasmid pRM12761
• Accession: NZ_CP007134
• Organism: Escherichia coli O145:H28 str. RM12761

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A6X6XTU7
• Locus tag: ECRM12761_RS27510
• Protein ID: WP_000702245.1
• Coordinates: 8472..8732 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: ECRM12761_RS27515
• Protein ID: WP_001132407.1
• Coordinates: 8722..8973 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECRM12761_RS27465	4068..4229	+	162	WP_001434039.1	type I toxin-antitoxin system Hok family toxin	-
ECRM12761_RS27470	4379..4975	+	597	WP_000125164.1	hypothetical protein	-
ECRM12761_RS33030	4993..5277	-	285	WP_001162371.1	hypothetical protein	-
ECRM12761_RS27480	5351..6016	-	666	WP_000532148.1	P-loop NTPase	-
ECRM12761_RS27485	6614..6943	+	330	WP_000855120.1	hypothetical protein	-
ECRM12761_RS27495	7525..7848	+	324	WP_000432876.1	hypothetical protein	-
ECRM12761_RS27500	7889..8098	+	210	WP_000866037.1	hypothetical protein	-
ECRM12761_RS27505	8196..8420	+	225	WP_000814384.1	hypothetical protein	-
ECRM12761_RS27510	8472..8732	+	261	WP_000702245.1	hypothetical protein	Toxin
ECRM12761_RS27515	8722..8973	+	252	WP_001132407.1	transcriptional regulator	Antitoxin
ECRM12761_RS27520	9523..9801	+	279	WP_025380728.1	hypothetical protein	-
ECRM12761_RS27525	10129..11337	+	1209	WP_051494565.1	transposase	-
ECRM12761_RS27530	11525..11707	-	183	WP_025380730.1	hypothetical protein	-
ECRM12761_RS27535	12265..12447	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
ECRM12761_RS27540	12472..12909	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
ECRM12761_RS27545	13040..13495	-	456	WP_000206705.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..58666	58666
	-	flank	IS/Tn	-	-	10189..11337	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9818.23 Da
• Isoelectric Point: 9.5163

>T46065 WP_000702245.1 NZ_CP007134:8472-8732 [Escherichia coli O145:H28 str. RM12761]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEEAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T46065 NZ_CP007134:8472-8732 [Escherichia coli O145:H28 str. RM12761]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGGAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT46065 WP_001132407.1 NZ_CP007134:8722-8973 [Escherichia coli O145:H28 str. RM12761]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT46065 NZ_CP007134:8722-8973 [Escherichia coli O145:H28 str. RM12761]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCTGAAAT
ATTCAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A6X6XTU7