Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 5236162..5236994 Replicon chromosome
Accession NZ_CP007133
Organism Escherichia coli O145:H28 str. RM12761

Toxin (Protein)


Gene name yeeV Uniprot ID S1PD64
Locus tag ECRM12761_RS26425 Protein ID WP_000854753.1
Coordinates 5236620..5236994 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID V0ULY5
Locus tag ECRM12761_RS26420 Protein ID WP_001315620.1
Coordinates 5236162..5236530 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM12761_RS26385 5232998..5233453 + 456 WP_000581504.1 hypothetical protein -
ECRM12761_RS26390 5233532..5233765 + 234 WP_001119729.1 DUF905 family protein -
ECRM12761_RS26395 5233865..5234683 + 819 WP_001234620.1 DUF945 domain-containing protein -
ECRM12761_RS26400 5234738..5235223 + 486 WP_000849588.1 antirestriction protein -
ECRM12761_RS26405 5235239..5235715 + 477 WP_001560709.1 RadC family protein -
ECRM12761_RS26410 5235778..5235999 + 222 WP_000692345.1 DUF987 domain-containing protein -
ECRM12761_RS26420 5236162..5236530 + 369 WP_001315620.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ECRM12761_RS26425 5236620..5236994 + 375 WP_000854753.1 TA system toxin CbtA family protein Toxin
ECRM12761_RS26430 5236991..5237479 + 489 WP_000777547.1 hypothetical protein -
ECRM12761_RS26435 5237491..5237688 + 198 WP_000839281.1 DUF957 domain-containing protein -
ECRM12761_RS32620 5237773..5237970 + 198 WP_025380687.1 hypothetical protein -
ECRM12761_RS26445 5237985..5238410 + 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
ECRM12761_RS26450 5238407..5238757 + 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
ECRM12761_RS26455 5238788..5240401 + 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - espL2 / nleB1 / nleE / efa1 / fimE / fimA / fimI / fimC 5195116..5250586 55470


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14005.00 Da        Isoelectric Point: 8.2905

>T46064 WP_000854753.1 NZ_CP007133:5236620-5236994 [Escherichia coli O145:H28 str. RM12761]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Download         Length: 375 bp

>T46064 NZ_CP007133:5236620-5236994 [Escherichia coli O145:H28 str. RM12761]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13474.20 Da        Isoelectric Point: 6.2050

>AT46064 WP_001315620.1 NZ_CP007133:5236162-5236530 [Escherichia coli O145:H28 str. RM12761]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT46064 NZ_CP007133:5236162-5236530 [Escherichia coli O145:H28 str. RM12761]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LVU0

Antitoxin

Source ID Structure
AlphaFold DB V0ULY5

References