Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4984011..4984508 Replicon chromosome
Accession NZ_CP007133
Organism Escherichia coli O145:H28 str. RM12761

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECRM12761_RS25200 Protein ID WP_001173343.1
Coordinates 4984335..4984508 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ECRM12761_RS25195 Protein ID WP_000398619.1
Coordinates 4984011..4984307 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM12761_RS25170 4980742..4981461 - 720 WP_000611288.1 two-component system response regulator DcuR -
ECRM12761_RS25175 4981458..4983089 - 1632 WP_022581427.1 sensor histidine kinase -
ECRM12761_RS25180 4983270..4983500 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
ECRM12761_RS25185 4983512..4983784 + 273 WP_000405653.1 N-acetyltransferase -
ECRM12761_RS25195 4984011..4984307 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECRM12761_RS25200 4984335..4984508 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECRM12761_RS25205 4984627..4986144 - 1518 WP_001295074.1 lysine--tRNA ligase -
ECRM12761_RS25210 4986381..4987838 - 1458 WP_000856822.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T46062 WP_001173343.1 NZ_CP007133:4984335-4984508 [Escherichia coli O145:H28 str. RM12761]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T46062 NZ_CP007133:4984335-4984508 [Escherichia coli O145:H28 str. RM12761]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT46062 WP_000398619.1 NZ_CP007133:4984011-4984307 [Escherichia coli O145:H28 str. RM12761]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT46062 NZ_CP007133:4984011-4984307 [Escherichia coli O145:H28 str. RM12761]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References