Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4198702..4199333 Replicon chromosome
Accession NZ_CP007133
Organism Escherichia coli O145:H28 str. RM12761

Toxin (Protein)


Gene name hicA Uniprot ID A0A7U9LJQ2
Locus tag ECRM12761_RS21430 Protein ID WP_001259387.1
Coordinates 4199058..4199333 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag ECRM12761_RS21425 Protein ID WP_000593555.1
Coordinates 4198702..4199061 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM12761_RS21395 4193814..4194287 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
ECRM12761_RS21400 4194284..4194565 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
ECRM12761_RS21405 4194642..4196000 + 1359 WP_001308116.1 PTS galactitol transporter subunit IIC -
ECRM12761_RS21410 4195993..4197501 + 1509 WP_001400448.1 carbohydrate kinase -
ECRM12761_RS21415 4197491..4197760 + 270 WP_000933061.1 HPr family phosphocarrier protein -
ECRM12761_RS21420 4197792..4198652 + 861 WP_001131788.1 class II aldolase -
ECRM12761_RS21425 4198702..4199061 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ECRM12761_RS21430 4199058..4199333 - 276 WP_001259387.1 type II toxin-antitoxin system HicA family toxin Toxin
ECRM12761_RS21435 4199406..4200530 - 1125 WP_025380616.1 ABC transporter permease -
ECRM12761_RS21440 4200530..4203265 - 2736 WP_000149086.1 ribosome-associated ATPase/putative transporter RbbA -
ECRM12761_RS21445 4203262..4204329 - 1068 WP_000361517.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4193043..4208168 15125


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10214.91 Da        Isoelectric Point: 10.7076

>T46061 WP_001259387.1 NZ_CP007133:c4199333-4199058 [Escherichia coli O145:H28 str. RM12761]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESQVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T46061 NZ_CP007133:c4199333-4199058 [Escherichia coli O145:H28 str. RM12761]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACAGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT46061 WP_000593555.1 NZ_CP007133:c4199061-4198702 [Escherichia coli O145:H28 str. RM12761]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT46061 NZ_CP007133:c4199061-4198702 [Escherichia coli O145:H28 str. RM12761]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAGCTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LJQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References