Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1782959..1783597 Replicon chromosome
Accession NZ_CP007133
Organism Escherichia coli O145:H28 str. RM12761

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ECRM12761_RS09050 Protein ID WP_000813794.1
Coordinates 1782959..1783135 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ECRM12761_RS09055 Protein ID WP_001270286.1
Coordinates 1783181..1783597 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM12761_RS09030 1778581..1779792 - 1212 WP_071792349.1 BenE family transporter YdcO -
ECRM12761_RS09035 1779845..1780381 + 537 WP_000429147.1 DNA-binding transcriptional regulator SutR -
ECRM12761_RS09040 1780454..1782415 + 1962 WP_025380370.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ECRM12761_RS09045 1782507..1782737 - 231 WP_000494244.1 YncJ family protein -
ECRM12761_RS09050 1782959..1783135 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ECRM12761_RS09055 1783181..1783597 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ECRM12761_RS09060 1783676..1785082 + 1407 WP_000760665.1 PLP-dependent aminotransferase family protein -
ECRM12761_RS09065 1785328..1786473 + 1146 WP_000335342.1 ABC transporter substrate-binding protein -
ECRM12761_RS09070 1786491..1787504 + 1014 WP_000220414.1 ABC transporter ATP-binding protein -
ECRM12761_RS09075 1787505..1788446 + 942 WP_001251328.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T46040 WP_000813794.1 NZ_CP007133:1782959-1783135 [Escherichia coli O145:H28 str. RM12761]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T46040 NZ_CP007133:1782959-1783135 [Escherichia coli O145:H28 str. RM12761]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT46040 WP_001270286.1 NZ_CP007133:1783181-1783597 [Escherichia coli O145:H28 str. RM12761]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT46040 NZ_CP007133:1783181-1783597 [Escherichia coli O145:H28 str. RM12761]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCATTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References