Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1300468..1301263 Replicon chromosome
Accession NZ_CP007133
Organism Escherichia coli O145:H28 str. RM12761

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag ECRM12761_RS06405 Protein ID WP_000854914.1
Coordinates 1300889..1301263 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A7U9LM11
Locus tag ECRM12761_RS06400 Protein ID WP_001280953.1
Coordinates 1300468..1300842 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRM12761_RS06360 1295823..1296728 + 906 WP_000203541.1 chemotaxis protein -
ECRM12761_RS06365 1296725..1297795 + 1071 WP_000102643.1 patatin-like phospholipase family protein -
ECRM12761_RS06375 1298135..1298953 + 819 WP_001234682.1 DUF945 domain-containing protein -
ECRM12761_RS06380 1299044..1299529 + 486 WP_000214398.1 antirestriction protein -
ECRM12761_RS06385 1299545..1300021 + 477 WP_001186738.1 RadC family protein -
ECRM12761_RS06390 1300084..1300305 + 222 WP_000692323.1 DUF987 domain-containing protein -
ECRM12761_RS06400 1300468..1300842 + 375 WP_001280953.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ECRM12761_RS06405 1300889..1301263 + 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
ECRM12761_RS06410 1301260..1301751 + 492 WP_144319763.1 hypothetical protein -
ECRM12761_RS06415 1301763..1301960 + 198 WP_000839282.1 DUF957 domain-containing protein -
ECRM12761_RS06420 1302045..1302887 + 843 WP_001280481.1 DUF4942 domain-containing protein -
ECRM12761_RS06430 1303357..1304295 + 939 WP_000351301.1 glyoxylate/hydroxypyruvate reductase GhrA -
ECRM12761_RS06435 1304350..1305087 + 738 WP_000283664.1 zinc-binding phosphatase -
ECRM12761_RS06440 1305111..1305665 + 555 WP_001001917.1 molecular chaperone YcdY -
ECRM12761_RS06445 1305767..1306258 + 492 WP_001297187.1 DUF1097 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - csgG / csgF / csgE / csgD / csgB / csgA / csgC 1288785..1310701 21916


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T46039 WP_000854914.1 NZ_CP007133:1300889-1301263 [Escherichia coli O145:H28 str. RM12761]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T46039 NZ_CP007133:1300889-1301263 [Escherichia coli O145:H28 str. RM12761]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13734.56 Da        Isoelectric Point: 6.6249

>AT46039 WP_001280953.1 NZ_CP007133:1300468-1300842 [Escherichia coli O145:H28 str. RM12761]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPALATTS

Download         Length: 375 bp

>AT46039 NZ_CP007133:1300468-1300842 [Escherichia coli O145:H28 str. RM12761]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACTCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LM11

References