Detailed information of TA system TA046032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 847589..848175
• Replicon: plasmid 1
• Accession: NZ_CP007129
• Organism: Gemmatirosa kalamazoonesis strain KBS708

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: J421_RS26845
• Protein ID: WP_025414206.1
• Coordinates: 847589..847918 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: J421_RS26850
• Protein ID: WP_025414207.1
• Coordinates: 847924..848175 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J421_RS32335	843076..843393	-	318	WP_148306566.1	hypothetical protein	-
J421_RS26810	843539..843853	-	315	WP_025414199.1	hypothetical protein	-
J421_RS33160	844295..844639	-	345	WP_025414200.1	YggL family protein	-
J421_RS33165	844764..845111	-	348	WP_025414201.1	hypothetical protein	-
J421_RS26820	845248..845541	-	294	WP_104023414.1	type II toxin-antitoxin system RelE/ParE family toxin	-
J421_RS26825	845556..845822	-	267	WP_025414203.1	addiction module protein	-
J421_RS26835	846423..846866	-	444	WP_025414204.1	hypothetical protein	-
J421_RS26840	847021..847407	-	387	WP_148306567.1	hypothetical protein	-
J421_RS26845	847589..847918	-	330	WP_025414206.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J421_RS26850	847924..848175	-	252	WP_025414207.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J421_RS26855	848433..848828	-	396	WP_158508917.1	DUF4440 domain-containing protein	-
J421_RS26860	849066..849356	+	291	WP_025414196.1	nucleotidyltransferase family protein	-
J421_RS26865	849394..849744	+	351	WP_025414209.1	DUF86 domain-containing protein	-
J421_RS26875	850224..850520	-	297	WP_025414210.1	hypothetical protein	-
J421_RS26880	850673..851071	-	399	WP_025414211.1	hypothetical protein	-
J421_RS26890	851610..852131	-	522	WP_025414213.1	hypothetical protein	-
J421_RS26895	852241..852840	-	600	WP_025414214.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1106233	1106233
	-	inside	Integron	-	-	813198..857786	44588

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-107)

Antitoxin

• SpoVT-AbrB domain (6-48)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12225.14 Da
• Isoelectric Point: 9.9269

>T46032 WP_025414206.1 NZ_CP007129:c847918-847589 [Gemmatirosa kalamazoonesis]
VRHAADRGAVYLVELDPTRGSEIRKTRPCVVVSPDELNHHLRTVIVAPMTTAGQPYPWRVPCRFRNKAGTVAVDQLRTVD
RERLVQRLGALPDATVLAVLERLTEFFAP

Nucleotide

• Length: 330 bp

>T46032 NZ_CP007129:c847918-847589 [Gemmatirosa kalamazoonesis]
GTGCGGCACGCGGCCGACCGCGGGGCCGTCTACCTCGTCGAGCTCGACCCGACGCGCGGGAGCGAGATCCGGAAGACGCG
GCCGTGCGTCGTGGTGTCGCCGGACGAGTTGAACCACCACCTGCGGACGGTAATCGTCGCGCCGATGACCACCGCGGGGC
AACCGTACCCGTGGCGCGTGCCGTGTCGGTTCCGCAATAAGGCGGGCACCGTCGCCGTAGATCAGCTCCGGACCGTGGAT
CGCGAGCGACTGGTGCAGCGGCTCGGCGCGCTGCCCGACGCGACGGTGCTCGCCGTGTTGGAGCGCCTCACCGAGTTCTT
CGCGCCGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9407.71 Da
• Isoelectric Point: 7.2068

>AT46032 WP_025414207.1 NZ_CP007129:c848175-847924 [Gemmatirosa kalamazoonesis]
MKARIVRVGNSRGIRIPKPLLDQAGLAEHVELHAEPGRIVIEAVKRPRDGWAEAARRMAARGDDALLDAPTPTRFDDEEW
EWR

Nucleotide

• Length: 252 bp

>AT46032 NZ_CP007129:c848175-847924 [Gemmatirosa kalamazoonesis]
ATGAAGGCCCGCATCGTCCGCGTCGGTAACTCGCGTGGCATCCGCATCCCGAAGCCCCTCCTGGACCAGGCGGGGCTCGC
TGAGCACGTCGAGCTTCATGCGGAGCCCGGCCGCATCGTCATCGAGGCGGTGAAGCGGCCGCGCGACGGCTGGGCGGAGG
CGGCGCGGCGGATGGCGGCGCGCGGCGACGATGCGCTGCTCGACGCGCCGACGCCGACGCGGTTCGACGACGAGGAGTGG
GAGTGGCGCTAA