Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2476706..2477357 Replicon chromosome
Accession NZ_CP007122
Organism Lactobacillus paracasei N1115

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag AF91_RS12250 Protein ID WP_003567661.1
Coordinates 2476706..2477089 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag AF91_RS12255 Protein ID WP_003567665.1
Coordinates 2477109..2477357 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AF91_RS12245 2476001..2476522 - 522 WP_003662697.1 QueT transporter family protein -
AF91_RS12250 2476706..2477089 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AF91_RS12255 2477109..2477357 - 249 WP_003567665.1 antitoxin Antitoxin
AF91_RS12260 2477425..2478561 - 1137 WP_003662696.1 alanine racemase -
AF91_RS12265 2478548..2478922 - 375 WP_003567669.1 holo-ACP synthase -
AF91_RS12270 2479092..2480600 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
AF91_RS17330 2480726..2480869 - 144 WP_003567673.1 hypothetical protein -
AF91_RS12280 2480900..2482288 - 1389 WP_003662693.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T46031 WP_003567661.1 NZ_CP007122:c2477089-2476706 [Lactobacillus paracasei N1115]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T46031 NZ_CP007122:c2477089-2476706 [Lactobacillus paracasei N1115]
ATGGATGTGTCCGTAAAACGCGGCGATGTTTTTTTCGCCGATCTGTCTCCGGTTGTCGGCTCCGAACAAGGCGGCAACCG
GCCAGTACTTATTATTCAAAACAACGTTGGCAACCATTACAGTCCCACAGTGATTGTGGCAGCCATTACCTCCAAGATTC
AAAAACCCAAGATGCCGACGCATGTCGGGTTACGGGCCAAGCAAGATGGCGTTGAACGCAACTCGGTCATTTTACTTGAG
CAAATTCGCACGATTGACAAGCAACGGCTACAAGCCCGCGTGACAGCTCTGTCAAGCACCAAAATGGCAGCAGTCGACCG
CGCGTTGGCGATTTCAGTGGGTCTGGTCAGTTTGCCCAAGCCCAAAACATATAATAAGAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT46031 WP_003567665.1 NZ_CP007122:c2477357-2477109 [Lactobacillus paracasei N1115]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT46031 NZ_CP007122:c2477357-2477109 [Lactobacillus paracasei N1115]
ATGAAAAAGCAAACGTCAGTAGCGGTAACGTTTGATGATGCCATTAAGGCGCGGTTGGATGCCTATTGTGAATTGAATGA
CATTGACGCTCAGGCTGTGATTACGGAGGCAGTGGCGACATTTTTGAAGTCACAGGCTCCCGAAATCAACCAATTGATTT
CTGGCTATGTAGCAATGGGCCAGATCAATACAGAGATCAGTCATGCTTTCTCTGCCTGCGAGAGCGAGGCATACGCCCAT
ATTCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References