Detailed information of TA system TA046029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-toxA/Tad-HTH_37
• Location: 233494..234122
• Replicon: chromosome
• Accession: NZ_CP007122
• Organism: Lactobacillus paracasei N1115

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A806L3G4
• Locus tag: AF91_RS01195
• Protein ID: WP_012490993.1
• Coordinates: 233754..234122 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: AF91_RS01190
• Protein ID: WP_003583264.1
• Coordinates: 233494..233754 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AF91_RS01165	229385..230128	+	744	WP_025375954.1	SDR family oxidoreductase	-
AF91_RS01170	230241..230867	+	627	WP_003577713.1	flavodoxin	-
AF91_RS01175	231118..231621	+	504	WP_012490991.1	TetR/AcrR family transcriptional regulator	-
AF91_RS01180	231626..232687	+	1062	WP_016365655.1	ABC transporter permease	-
AF91_RS01185	232692..233381	+	690	WP_003563376.1	ABC transporter ATP-binding protein	-
AF91_RS01190	233494..233754	-	261	WP_003583264.1	helix-turn-helix transcriptional regulator	Antitoxin
AF91_RS01195	233754..234122	-	369	WP_012490993.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AF91_RS01200	234413..235783	-	1371	WP_016365454.1	FAD-dependent oxidoreductase	-
AF91_RS01205	236147..236965	-	819	WP_011674103.1	Cof-type HAD-IIB family hydrolase	-
AF91_RS01210	237281..237550	-	270	WP_016364183.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
AF91_RS01215	237791..238798	+	1008	WP_016365455.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (34-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-79)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 14970.02 Da
• Isoelectric Point: 8.3986

>T46029 WP_012490993.1 NZ_CP007122:c234122-233754 [Lactobacillus paracasei N1115]
MYKIDFYEDRDGYSEIEDFLDQLRHSHQKNNVSLLNKITKELYMLQNLGPRLREPHAKFLKGYAYPIMELRPMQERIFYA
AWQKDHFVLLHHYTKKTNKTDHREILRAQSNLEDWLSRKEEN

Nucleotide

• Length: 369 bp

>T46029 NZ_CP007122:c234122-233754 [Lactobacillus paracasei N1115]
ATGTATAAAATTGACTTCTATGAGGATCGCGATGGCTACTCTGAGATCGAAGACTTTCTTGATCAGTTACGCCATAGTCA
TCAAAAAAATAATGTGAGCCTTTTAAACAAAATCACAAAGGAACTCTACATGCTGCAAAATCTTGGTCCGCGCTTACGTG
AACCTCACGCGAAGTTTCTGAAGGGATATGCTTATCCGATCATGGAATTGCGTCCCATGCAAGAAAGAATCTTTTATGCT
GCATGGCAAAAGGATCATTTCGTTTTGCTTCATCACTACACCAAGAAAACAAACAAAACTGATCACAGAGAAATTTTACG
GGCACAGTCTAACCTCGAAGATTGGCTCTCTAGAAAGGAAGAAAATTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9828.45 Da
• Isoelectric Point: 10.2439

>AT46029 WP_003583264.1 NZ_CP007122:c233754-233494 [Lactobacillus paracasei N1115]
MTTWEDFSQKLKKDNPEKLAIIKTLADLESERIKQGISQKDFAEKIGMKQPQLAKIERLDSLPTLATLNRYARGLGKEIR
LSIVTQ

Nucleotide

• Length: 261 bp

>AT46029 NZ_CP007122:c233754-233494 [Lactobacillus paracasei N1115]
ATGACGACATGGGAAGACTTCAGTCAGAAACTGAAGAAGGACAATCCAGAAAAGTTGGCAATCATCAAAACCTTGGCCGA
TTTAGAAAGTGAACGAATCAAGCAAGGAATCTCTCAAAAGGACTTTGCAGAAAAAATTGGTATGAAACAGCCGCAACTAG
CAAAGATTGAGCGCTTAGACTCTCTTCCGACTCTGGCAACACTCAATCGATACGCGAGAGGGCTTGGAAAAGAAATTCGT
TTATCAATTGTTACTCAGTGA

Structures

Toxin

• AlphaFold DB: A0A806L3G4