Detailed information of TA system TA046027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 509570..510177
• Replicon: plasmid unnamed
• Accession: NZ_CP007068
• Organism: Rhizobium leguminosarum bv. trifolii CB782

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: J0VFB1
• Locus tag: RLEG12_RS23445
• Protein ID: WP_003572077.1
• Coordinates: 509570..509824 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: RLEG12_RS23450
• Protein ID: WP_025417959.1
• Coordinates: 509821..510177 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLEG12_RS23430	505017..505217	+	201	WP_003572080.1	hypothetical protein	-
RLEG12_RS23435	505625..506857	+	1233	WP_025417957.1	HlyD family efflux transporter periplasmic adaptor subunit	-
RLEG12_RS23440	506839..508833	+	1995	WP_025417958.1	glycosyltransferase	-
RLEG12_RS23445	509570..509824	+	255	WP_003572077.1	type II toxin-antitoxin system HicA family toxin	Toxin
RLEG12_RS23450	509821..510177	+	357	WP_025417959.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
RLEG12_RS23455	510381..511442	-	1062	WP_052349981.1	hypothetical protein	-
RLEG12_RS23460	511762..512358	-	597	WP_003572073.1	TetR/AcrR family transcriptional regulator	-
RLEG12_RS23465	512450..513541	+	1092	WP_155256657.1	saccharopine dehydrogenase NADP-binding domain-containing protein	-
RLEG12_RS23470	513696..514739	-	1044	WP_025417962.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	gmd / htpB	1..1563772	1563772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9336.70 Da
• Isoelectric Point: 9.8124

>T46027 WP_003572077.1 NZ_CP007068:509570-509824 [Rhizobium leguminosarum bv. trifolii CB782]
MNSKQRKTLAAVFKDPVSGTIEWAAIENLLVAAGADLIEGNGSRVKFEKDGIIASFHRPHPDKEAKRYQVRDAREFLMQI
GVTP

Nucleotide

• Length: 255 bp

>T46027 NZ_CP007068:509570-509824 [Rhizobium leguminosarum bv. trifolii CB782]
ATGAACAGCAAGCAGAGAAAGACTCTCGCGGCGGTCTTCAAGGACCCAGTCTCGGGAACCATTGAATGGGCCGCTATAGA
GAATCTGCTTGTAGCTGCCGGAGCCGACCTCATTGAGGGGAACGGTTCCCGCGTTAAGTTTGAAAAAGACGGGATCATTG
CGAGCTTCCACAGACCACATCCGGACAAGGAAGCAAAGCGTTATCAGGTGCGCGATGCTCGCGAGTTCCTGATGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13288.93 Da
• Isoelectric Point: 4.9838

>AT46027 WP_025417959.1 NZ_CP007068:509821-510177 [Rhizobium leguminosarum bv. trifolii CB782]
MNIMSYNGYHARIEFDAEDDVFFGRIAGISDIIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLEEAADHYAEARLPA

Nucleotide

• Length: 357 bp

>AT46027 NZ_CP007068:509821-510177 [Rhizobium leguminosarum bv. trifolii CB782]
ATGAACATCATGAGCTACAACGGATATCACGCTCGCATCGAATTCGACGCTGAGGACGATGTGTTTTTCGGCAGGATTGC
AGGTATCTCAGATATAATCGGCTTTCACGGTGACAGTGTCGCTGAACTCAAGAAAGCCTTCCATGAGGCAGTCGATGACT
ACCTGGAGACCTGTAGGAAAATCGGCAAAGAGCCTCAGCGACCCTATTCTGGGAAGATGATGTTTCGTGTCGCTCCCGAA
GTCCATCGCCGAGCAGCTCTTGCTGCTGAGCTTTCGGGAAAGAGTCTGAACCAGTGGGCAGAAGAGGTGCTGGAAGAGGC
CGCCGACCATTATGCCGAGGCGCGCCTGCCGGCGTAG

Structures

Toxin

• AlphaFold DB: A0A246TAE7