Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/PRK09907-MazE
Location 2170459..2171030 Replicon chromosome
Accession NZ_CP007062
Organism Fusobacterium nucleatum subsp. animalis 7_1

Toxin (Protein)


Gene name mazF Uniprot ID A0A140PSE3
Locus tag FSDG_RS10290 Protein ID WP_008701956.1
Coordinates 2170459..2170782 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A140PUK7
Locus tag FSDG_RS10295 Protein ID WP_008701954.1
Coordinates 2170776..2171030 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FSDG_RS10255 2165597..2166577 + 981 WP_008701963.1 polyprenyl synthetase family protein -
FSDG_RS10260 2166574..2167497 + 924 WP_008701962.1 prenyltransferase -
FSDG_RS10265 2167499..2168194 + 696 WP_008701961.1 ubiquinone/menaquinone biosynthesis methyltransferase -
FSDG_RS10270 2168218..2169513 + 1296 WP_008701960.1 FAD-dependent oxidoreductase -
FSDG_RS10275 2169516..2169800 + 285 WP_005910587.1 ferredoxin family protein -
FSDG_RS10280 2169854..2170174 - 321 WP_008701959.1 type II toxin-antitoxin system YoeB family toxin -
FSDG_RS10285 2170167..2170424 - 258 WP_008701957.1 hypothetical protein -
FSDG_RS10290 2170459..2170782 - 324 WP_008701956.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FSDG_RS10295 2170776..2171030 - 255 WP_008701954.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FSDG_RS10300 2171232..2173298 - 2067 WP_016361475.1 elongation factor G -
FSDG_RS10305 2173385..2174854 - 1470 WP_008701950.1 N-acetylglucosamine-specific PTS transporter subunit IIBC -
FSDG_RS10310 2175096..2175515 + 420 WP_008701948.1 NfeD family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-106)

Antitoxin

SpoVT-AbrB domain

(7-49)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12437.59 Da        Isoelectric Point: 8.0539

>T46020 WP_008701956.1 NZ_CP007062:c2170782-2170459 [Fusobacterium nucleatum subsp. animalis 7_1]
VVERGDIIIINFDPVKGHEQAGKRPALVISNEKFYRIFKLAVILPITNNTKDFPFHVLLDDRTNIKGVILCEHLRTVDLE
ERKYNKVEKLPKNLLEEVLEKVSLIFQ

Download         Length: 324 bp

>T46020 NZ_CP007062:c2170782-2170459 [Fusobacterium nucleatum subsp. animalis 7_1]
GTGGTAGAAAGAGGAGATATTATTATAATAAATTTTGATCCTGTAAAAGGACATGAACAAGCAGGGAAAAGGCCAGCATT
GGTTATCAGTAATGAGAAATTTTATAGAATATTTAAATTAGCAGTAATTCTTCCAATAACAAATAATACAAAAGATTTTC
CATTTCACGTGCTATTAGATGATAGAACAAATATAAAAGGTGTAATATTATGTGAACATTTAAGAACAGTTGATTTAGAA
GAAAGAAAATACAATAAAGTTGAAAAATTACCAAAAAATCTGTTAGAAGAAGTTCTTGAAAAAGTGAGCTTAATTTTTCA
ATAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 10117.64 Da        Isoelectric Point: 8.4038

>AT46020 WP_008701954.1 NZ_CP007062:c2171030-2170776 [Fusobacterium nucleatum subsp. animalis 7_1]
MEVLRVQKWGNSQGIRLPKKILKDLKIDINDKIEISLNGEEIILKKVKKYTSLDDLFKDYRGDYRKDFDEFEFFGEAQGR
EFKW

Download         Length: 255 bp

>AT46020 NZ_CP007062:c2171030-2170776 [Fusobacterium nucleatum subsp. animalis 7_1]
ATGGAAGTATTAAGAGTTCAAAAATGGGGAAATAGTCAAGGAATTAGATTACCTAAAAAAATTTTAAAAGATTTAAAAAT
AGATATAAATGATAAAATTGAAATAAGTTTAAATGGAGAAGAAATTATACTAAAAAAAGTAAAAAAATATACTAGTTTAG
ATGATTTATTTAAAGATTATAGAGGAGACTATAGAAAAGATTTTGATGAATTTGAATTTTTTGGAGAAGCACAAGGTCGT
GAATTTAAGTGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140PSE3

Antitoxin

Source ID Structure
AlphaFold DB A0A140PUK7

References