Detailed information of TA system TA046019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 135815..136482
• Replicon: plasmid unnamed3
• Accession: NZ_CP007048
• Organism: Rhizobium leguminosarum bv. trifolii WSM1689

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: W0IUA5
• Locus tag: RLEG3_RS29630
• Protein ID: WP_025397970.1
• Coordinates: 136039..136482 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: RLEG3_RS29625
• Protein ID: WP_003555338.1
• Coordinates: 135815..136042 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLEG3_RS29610	131883..132797	-	915	WP_025397965.1	LysR family transcriptional regulator	-
RLEG3_RS29615	132933..133841	+	909	WP_025397966.1	LysR family transcriptional regulator	-
RLEG3_RS29620	134405..135478	+	1074	WP_025397968.1	zinc-binding dehydrogenase	-
RLEG3_RS29625	135815..136042	+	228	WP_003555338.1	hypothetical protein	Antitoxin
RLEG3_RS29630	136039..136482	+	444	WP_025397970.1	PIN domain-containing protein	Toxin
RLEG3_RS29635	136765..138192	-	1428	WP_025397971.1	ISNCY family transposase	-
RLEG3_RS29640	138775..140943	-	2169	WP_025397972.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..341391	341391
	-	flank	IS/Tn	-	-	136765..138192	1427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-131)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 15581.80 Da
• Isoelectric Point: 7.4219

>T46019 WP_025397970.1 NZ_CP007048:136039-136482 [Rhizobium leguminosarum bv. trifolii WSM1689]
VTFLLDVNVLIALIDPGHVAHDDAHEWFAAIGQSAWASCPITENGVIRIVGNPKYPNSPGSPSLVMEIVRKLRSLPGHSF
WPDDVSLVGSGDIAPTKILTSGQVTDTYLLALAKARGGQLATFDRKLSAAAVTRGNSALHLITTSRS

Nucleotide

• Length: 444 bp

>T46019 NZ_CP007048:136039-136482 [Rhizobium leguminosarum bv. trifolii WSM1689]
GTGACTTTCCTGCTCGACGTCAATGTGCTGATTGCCTTAATCGATCCGGGCCATGTAGCCCATGATGACGCGCATGAATG
GTTCGCGGCGATAGGTCAGAGCGCGTGGGCTAGCTGCCCCATTACCGAAAATGGTGTCATCCGGATTGTCGGCAACCCCA
AATATCCCAACTCTCCCGGTTCACCGTCACTCGTGATGGAAATTGTCCGCAAATTGCGGTCGCTCCCCGGTCACAGTTTT
TGGCCGGACGATGTGAGCCTTGTCGGGTCAGGTGATATCGCTCCCACGAAAATTCTAACATCGGGGCAGGTAACTGACAC
CTACCTGCTTGCGCTAGCCAAGGCGCGTGGGGGCCAGCTGGCAACATTCGATCGCAAGCTGTCAGCAGCAGCGGTTACAA
GGGGCAATTCTGCCTTGCACCTGATCACCACGAGTAGATCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8220.39 Da
• Isoelectric Point: 7.0120

>AT46019 WP_003555338.1 NZ_CP007048:135815-136042 [Rhizobium leguminosarum bv. trifolii WSM1689]
MRTTLAIDDDVLTAAKAMATQQHRSVGEVISELARRSLRRPPSSGERNGIPLLSTRPDTPPVTLEIVNAMRDELP

Nucleotide

• Length: 228 bp

>AT46019 NZ_CP007048:135815-136042 [Rhizobium leguminosarum bv. trifolii WSM1689]
ATGAGAACGACTTTGGCAATCGACGACGATGTGCTGACCGCCGCAAAAGCGATGGCAACCCAGCAGCACCGGAGCGTCGG
CGAGGTCATTTCTGAGCTGGCGAGACGTTCTCTGCGCCGACCGCCGAGCAGCGGCGAGCGCAATGGCATTCCTCTCCTGT
CAACACGGCCGGACACGCCGCCAGTGACGCTCGAAATCGTGAACGCCATGCGTGACGAACTGCCGTGA

Structures

Toxin

• AlphaFold DB: W0IUA5