Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 67714..68390 Replicon plasmid unnamed
Accession NZ_CP007046
Organism Rhizobium leguminosarum bv. trifolii WSM1689

Toxin (Protein)


Gene name vapC Uniprot ID W0IQM2
Locus tag RLEG3_RS23825 Protein ID WP_025396901.1
Coordinates 67714..68139 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1S9GB48
Locus tag RLEG3_RS33905 Protein ID WP_065282799.1
Coordinates 68136..68390 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RLEG3_RS33900 63061..63720 - 660 WP_080685846.1 sigma-70 family RNA polymerase sigma factor -
RLEG3_RS23810 63884..64375 + 492 WP_025396898.1 GNAT family N-acetyltransferase -
RLEG3_RS23815 64463..65239 - 777 WP_025396899.1 siderophore-iron reductase FhuF -
RLEG3_RS23820 65244..67415 - 2172 WP_025396900.1 TonB-dependent siderophore receptor -
RLEG3_RS23825 67714..68139 - 426 WP_025396901.1 type II toxin-antitoxin system VapC family toxin Toxin
RLEG3_RS33905 68136..68390 - 255 WP_065282799.1 plasmid stabilization protein Antitoxin
RLEG3_RS23830 68847..70064 + 1218 WP_041472577.1 GGDEF domain-containing protein -
RLEG3_RS23835 70243..70986 + 744 WP_025396903.1 FadR family transcriptional regulator -
RLEG3_RS23840 70970..72568 + 1599 WP_025396904.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..667306 667306


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15355.64 Da        Isoelectric Point: 4.8146

>T46017 WP_025396901.1 NZ_CP007046:c68139-67714 [Rhizobium leguminosarum bv. trifolii WSM1689]
MILIDTNVISEPWKPTPEYQVLAWIDAQAVETLFVSTITVAELRFGIAAMPSGRRKTVLYDRLEQEVLPLFSGRVLSFDM
AASQSYSELMARARLAGRAIGKADAYIAATAAANGFIVATRDSSPFEAAGLNVINPWYAPT

Download         Length: 426 bp

>T46017 NZ_CP007046:c68139-67714 [Rhizobium leguminosarum bv. trifolii WSM1689]
ATGATCCTGATCGATACCAACGTCATCTCGGAGCCATGGAAGCCCACACCCGAATATCAGGTGCTGGCGTGGATCGATGC
TCAAGCAGTGGAAACCCTGTTCGTTTCGACGATTACAGTAGCTGAGTTGCGGTTCGGCATTGCCGCGATGCCTAGCGGAA
GACGAAAGACCGTCCTCTACGATCGACTAGAACAAGAAGTTCTGCCTCTATTTTCAGGGCGCGTTCTATCCTTCGACATG
GCTGCGTCACAGAGCTATTCGGAATTGATGGCGCGAGCACGCCTAGCAGGTCGGGCAATCGGTAAAGCAGATGCCTATAT
CGCCGCAACTGCCGCTGCGAACGGATTTATTGTCGCCACCCGCGACAGTAGTCCCTTCGAAGCCGCAGGACTTAACGTTA
TCAACCCGTGGTACGCACCAACATAG

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9192.38 Da        Isoelectric Point: 6.2221

>AT46017 WP_065282799.1 NZ_CP007046:c68390-68136 [Rhizobium leguminosarum bv. trifolii WSM1689]
MAAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDILETAVRPAERVMLGSLLASIGREAGLSNNDVDNLQLVRDKAAAEP
MSFE

Download         Length: 255 bp

>AT46017 NZ_CP007046:c68390-68136 [Rhizobium leguminosarum bv. trifolii WSM1689]
ATGGCTGCAGTTACAATACGCAATTTGTCTGACGAGACACACCGTGCTTTAAGAGTGAGGGCAGCTCATCACGGCCGCAG
TACAGAAGCAGAAATCCGTGACATCCTTGAAACAGCGGTACGGCCAGCTGAGCGCGTCATGTTGGGCTCGTTGCTAGCAT
CGATCGGCCGTGAGGCAGGACTTTCCAACAACGACGTGGATAATCTGCAGCTAGTCCGAGACAAAGCAGCTGCGGAACCT
ATGAGTTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W0IQM2

Antitoxin

Source ID Structure
AlphaFold DB A0A1S9GB48

References