Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 6743..7545 Replicon plasmid unnamed
Accession NZ_CP007046
Organism Rhizobium leguminosarum bv. trifolii WSM1689

Toxin (Protein)


Gene name TacT3 Uniprot ID A0A1S9GBD6
Locus tag RLEG3_RS23555 Protein ID WP_025396848.1
Coordinates 6743..7270 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name TacA3 Uniprot ID W0IPV9
Locus tag RLEG3_RS23560 Protein ID WP_025396849.1
Coordinates 7267..7545 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RLEG3_RS23545 3363..4487 - 1125 WP_025396846.1 DNA repair exonuclease -
RLEG3_RS23550 4805..6451 - 1647 WP_025396847.1 acetolactate synthase large subunit -
RLEG3_RS23555 6743..7270 - 528 WP_025396848.1 GNAT family N-acetyltransferase Toxin
RLEG3_RS23560 7267..7545 - 279 WP_025396849.1 DUF1778 domain-containing protein Antitoxin
RLEG3_RS23565 7766..8644 - 879 WP_025396850.1 hypothetical protein -
RLEG3_RS23570 9059..9781 - 723 WP_025396851.1 ribonuclease activity regulator RraA -
RLEG3_RS23575 9852..10637 - 786 WP_025396852.1 IclR family transcriptional regulator -
RLEG3_RS23580 10852..12372 + 1521 WP_025396853.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..667306 667306


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Vibrio phage ICP1, Orf50

(12-87)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 18773.72 Da        Isoelectric Point: 9.5507

>T46016 WP_025396848.1 NZ_CP007046:c7270-6743 [Rhizobium leguminosarum bv. trifolii WSM1689]
VTLPVWHEEPIAKSHDRASFDCGDGAMNEFIRRFARQSHEQNAAKTFCAIDKAQPDRILGFYTVAPSAVTHEAVPPKLTR
GLARHEVAGFKLARLATDIKVAGKGLGGQLIAAAALRCLRLASEGGGILLIIDAKSERAAHWYASYGAEPLQGKPLTLVM
PLATFAADFKAKGLL

Download         Length: 528 bp

>T46016 NZ_CP007046:c7270-6743 [Rhizobium leguminosarum bv. trifolii WSM1689]
GTGACCCTCCCCGTCTGGCATGAAGAGCCGATCGCTAAGTCGCACGATCGGGCCTCATTCGATTGCGGCGATGGGGCAAT
GAACGAGTTCATTCGGCGCTTCGCTCGGCAGAGCCATGAGCAGAATGCGGCGAAGACCTTCTGCGCCATCGACAAGGCCC
AACCTGATCGCATCCTCGGATTCTACACAGTCGCGCCTTCGGCCGTTACACATGAGGCCGTGCCTCCAAAGCTGACGAGA
GGCCTCGCACGCCATGAAGTCGCAGGCTTCAAGCTTGCGCGCTTGGCAACCGACATAAAGGTGGCGGGAAAGGGTTTAGG
CGGCCAGCTCATCGCTGCTGCAGCGCTCCGTTGCCTGCGGCTCGCCAGCGAGGGCGGCGGGATCCTGCTCATCATCGATG
CCAAGAGCGAACGCGCCGCCCATTGGTATGCCAGCTATGGCGCCGAACCGCTGCAAGGGAAACCACTCACCCTCGTTATG
CCGCTTGCCACCTTCGCCGCCGACTTCAAGGCCAAGGGGCTCTTGTAG

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10171.84 Da        Isoelectric Point: 8.6184

>AT46016 WP_025396849.1 NZ_CP007046:c7545-7267 [Rhizobium leguminosarum bv. trifolii WSM1689]
MPRTADNKTERYQLRIPPKQKAIIARAAALSNKDMADFILDKIVPEAEAVIQAAEVETVSSRDFIRILDLLEHPPKPNAK
LRAAISALPDAL

Download         Length: 279 bp

>AT46016 NZ_CP007046:c7545-7267 [Rhizobium leguminosarum bv. trifolii WSM1689]
ATGCCGAGAACCGCGGACAACAAAACCGAACGCTACCAGCTCCGTATCCCGCCAAAGCAGAAGGCAATTATCGCGCGCGC
GGCTGCCCTCTCGAACAAGGATATGGCAGATTTCATTCTCGATAAGATAGTACCTGAGGCCGAAGCCGTCATCCAGGCTG
CTGAAGTTGAGACCGTGTCGAGCCGCGATTTCATCCGCATCCTCGACCTGCTTGAACACCCGCCAAAACCGAACGCGAAA
CTACGCGCTGCGATCTCTGCTCTACCGGATGCCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1S9GBD6

Antitoxin

Source ID Structure
AlphaFold DB A0A1S9GBB9

References