Detailed information of TA system TA046015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2850189..2850862
• Replicon: chromosome
• Accession: NZ_CP007045
• Organism: Rhizobium leguminosarum bv. trifolii WSM1689

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: W0IF99
• Locus tag: RLEG3_RS14120
• Protein ID: WP_025395284.1
• Coordinates: 2850189..2850602 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: W0IK01
• Locus tag: RLEG3_RS14125
• Protein ID: WP_025395285.1
• Coordinates: 2850599..2850862 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLEG3_RS14110	2847620..2848828	-	1209	WP_025395282.1	efflux RND transporter periplasmic adaptor subunit	-
RLEG3_RS14115	2849096..2850010	-	915	WP_025395283.1	adenylate/guanylate cyclase domain-containing protein	-
RLEG3_RS14120	2850189..2850602	-	414	WP_025395284.1	type II toxin-antitoxin system VapC family toxin	Toxin
RLEG3_RS14125	2850599..2850862	-	264	WP_025395285.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RLEG3_RS14130	2850951..2852501	-	1551	WP_025395286.1	SpoVR family protein	-
RLEG3_RS14135	2852498..2853772	-	1275	WP_025395287.1	YeaH/YhbH family protein	-
RLEG3_RS14140	2853785..2855728	-	1944	WP_025395288.1	PrkA family serine protein kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (9-54)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15228.59 Da
• Isoelectric Point: 5.2130

>T46015 WP_025395284.1 NZ_CP007045:c2850602-2850189 [Rhizobium leguminosarum bv. trifolii WSM1689]
MTAWMLDTNMVSHVIKGDRPEIIDRLAALPMEDIVISSITEGELFYGLAKRGYPKVLSERIRQFLLRVDVLPWDHDVTRN
YGDLRTACEAKGVTLAPLDMMIAAHAVATAATLVTRDKAFARVSEPLKVDDWASEGS

Nucleotide

• Length: 414 bp

>T46015 NZ_CP007045:c2850602-2850189 [Rhizobium leguminosarum bv. trifolii WSM1689]
ATGACGGCCTGGATGCTCGATACCAATATGGTGAGCCATGTCATCAAGGGCGATCGGCCGGAGATCATCGACAGGTTGGC
CGCGCTTCCGATGGAAGACATCGTCATCTCATCGATCACGGAAGGGGAGCTGTTCTACGGTCTTGCCAAGCGAGGCTATC
CGAAGGTGCTCTCGGAACGGATCCGCCAATTCCTGCTCCGGGTCGACGTTCTCCCTTGGGATCACGATGTCACGCGAAAT
TATGGCGACCTGCGCACTGCCTGTGAAGCCAAAGGCGTGACTCTGGCGCCCCTCGATATGATGATTGCCGCTCATGCCGT
TGCGACTGCCGCAACCCTCGTCACGCGCGACAAGGCTTTTGCTCGCGTTTCGGAGCCTTTGAAGGTCGATGACTGGGCGA
GTGAAGGAAGCTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10174.27 Da
• Isoelectric Point: 4.8948

>AT46015 WP_025395285.1 NZ_CP007045:c2850862-2850599 [Rhizobium leguminosarum bv. trifolii WSM1689]
MATRTAKIFTTGRSQAVRLPAEFRFEESEVFVRRDPKTGDVILSRKPDSWDGLFELYSKDQVPDDFLGPDDRRQPSHDRD
PFEGWKE

Nucleotide

• Length: 264 bp

>AT46015 NZ_CP007045:c2850862-2850599 [Rhizobium leguminosarum bv. trifolii WSM1689]
ATGGCAACGAGAACAGCCAAGATTTTCACGACCGGCCGCAGCCAGGCAGTCAGGCTCCCTGCGGAGTTCCGCTTCGAGGA
GAGTGAGGTGTTCGTCCGTCGTGATCCGAAGACCGGCGATGTCATTCTGTCACGCAAGCCGGATTCGTGGGATGGTCTCT
TTGAGCTTTATAGCAAGGATCAGGTTCCGGACGATTTCCTGGGGCCAGACGATCGCCGCCAGCCTTCGCATGACCGTGAT
CCATTCGAGGGTTGGAAGGAATGA

Structures

Toxin

• AlphaFold DB: W0IF99

Antitoxin

• AlphaFold DB: W0IK01