Detailed information of TA system TA046014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 815013..816502
• Replicon: chromosome
• Accession: NZ_CP007045
• Organism: Rhizobium leguminosarum bv. trifolii WSM1689

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: W0IE92
• Locus tag: RLEG3_RS03805
• Protein ID: WP_025393535.1
• Coordinates: 815013..816275 (-)
• Length: 421 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: RLEG3_RS33245
• Protein ID: WP_038613852.1
• Coordinates: 816272..816502 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLEG3_RS03785	810889..812316	-	1428	WP_025393531.1	ATP-binding protein	-
RLEG3_RS03790	812405..813490	+	1086	WP_025393532.1	DNA cytosine methyltransferase	-
RLEG3_RS33240	813493..813933	+	441	WP_080685780.1	DNA mismatch endonuclease Vsr	-
RLEG3_RS03800	814091..814870	-	780	WP_025393534.1	SDR family NAD(P)-dependent oxidoreductase	-
RLEG3_RS03805	815013..816275	-	1263	WP_025393535.1	type II toxin-antitoxin system HipA family toxin	Toxin
RLEG3_RS33245	816272..816502	-	231	WP_038613852.1	helix-turn-helix transcriptional regulator	Antitoxin
RLEG3_RS03815	817124..817732	-	609	WP_025393537.1	TetR/AcrR family transcriptional regulator	-
RLEG3_RS03820	817843..818529	+	687	WP_025393538.1	PspA/IM30 family protein	-
RLEG3_RS03825	818579..818860	+	282	WP_003569708.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (6-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 421 a.a.
• Molecular weight: 45584.43 Da
• Isoelectric Point: 5.5370

>T46014 WP_025393535.1 NZ_CP007045:c816275-815013 [Rhizobium leguminosarum bv. trifolii WSM1689]
MTSRLLDVWWDGRIVGQFTQDRHGDIGFVYARAWLDDDKTLPLSASLPKRPERFTRRECRPFFGGLLPEESQRLAAARAL
GVSPANDFALLDHLGGDVAGALQLLAEGEAPAETGSIADRQPVPLDEAGIVAVLEALPVRPLLAGEEGLRLSLAGAQSKL
PVVLVDGWIGLPLPGQATTHILKPPIARFKATTENEAFVMRLAAAIGLDVAAVEPRYVQERPFLLVERYDRVRGAGGLVH
RIHQEDFCQALSVPPETKYASEGGPTFKDCFDLIRRISARPAVDVLKLLDAAIFNLIAGNADAHGKNFSILYDTQGPRLA
KLYDLLSTVIYPELSPKLAMRLGKRATLAEMDAEGWKAFAAEAGVGYPLLRRRVNELCDKVLTLTGKVADELADVSPDRD
PIDQIAGLIAGRAEAARLTL

Nucleotide

• Length: 1263 bp

>T46014 NZ_CP007045:c816275-815013 [Rhizobium leguminosarum bv. trifolii WSM1689]
ATGACATCAAGGCTTCTCGACGTCTGGTGGGACGGCCGCATTGTCGGTCAATTCACGCAGGACAGACACGGCGATATCGG
CTTTGTCTATGCGCGAGCCTGGCTTGACGATGACAAAACGCTGCCGCTCTCGGCCTCTCTGCCGAAACGACCGGAGCGAT
TTACCCGCCGGGAATGCCGGCCGTTTTTCGGCGGCCTGCTGCCCGAAGAAAGCCAGCGCCTGGCCGCAGCAAGGGCCTTG
GGCGTGTCTCCCGCCAATGACTTTGCGCTTCTCGATCACCTGGGCGGCGATGTCGCCGGTGCGCTGCAACTGCTTGCCGA
AGGTGAGGCACCGGCCGAGACGGGGTCGATTGCAGATCGCCAGCCCGTGCCCCTGGACGAAGCGGGCATCGTTGCCGTGT
TGGAGGCACTGCCCGTGCGGCCTCTCCTGGCCGGTGAGGAAGGCCTCAGACTGTCGCTCGCCGGCGCGCAATCCAAACTG
CCGGTTGTTCTGGTCGATGGCTGGATCGGGCTTCCCCTGCCCGGTCAGGCCACAACCCATATCCTGAAGCCACCGATAGC
CCGCTTCAAGGCGACGACCGAGAACGAAGCTTTCGTGATGCGGCTTGCGGCGGCCATCGGGCTCGATGTCGCAGCCGTCG
AACCGAGATATGTGCAGGAACGCCCGTTCCTTCTGGTCGAACGATATGACCGCGTGCGCGGCGCCGGCGGACTGGTTCAT
CGCATCCATCAGGAAGATTTCTGCCAGGCGCTCAGCGTACCGCCGGAAACGAAATATGCGAGTGAAGGCGGGCCGACCTT
CAAGGATTGCTTCGATCTCATAAGACGGATCTCGGCAAGGCCGGCTGTCGATGTGCTGAAGCTGCTGGACGCGGCCATCT
TCAACCTGATCGCCGGAAATGCAGATGCCCACGGCAAGAACTTCTCCATCCTGTACGATACCCAAGGCCCGCGGCTGGCA
AAACTCTATGACCTCCTCTCCACCGTGATCTATCCGGAACTGTCGCCAAAGCTGGCGATGAGATTAGGCAAGCGCGCCAC
ACTGGCCGAAATGGATGCCGAGGGCTGGAAGGCCTTTGCGGCAGAAGCCGGCGTCGGCTATCCGCTGTTGCGCCGGCGCG
TGAACGAGCTTTGCGACAAGGTGCTGACATTGACCGGCAAGGTCGCCGATGAGCTCGCAGACGTATCGCCTGATCGGGAC
CCGATCGACCAGATCGCCGGGTTGATTGCGGGCCGGGCCGAGGCCGCGCGCTTGACTTTATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8159.46 Da
• Isoelectric Point: 9.3464

>AT46014 WP_038613852.1 NZ_CP007045:c816502-816272 [Rhizobium leguminosarum bv. trifolii WSM1689]
MPKHVQSPADIGALVRSTRKEQNLRQDELAGVAGVGLRFIVDLEAGKPTAQIGKVLQVLQTLGCSLDILAPGERRQ

Nucleotide

• Length: 231 bp

>AT46014 NZ_CP007045:c816502-816272 [Rhizobium leguminosarum bv. trifolii WSM1689]
ATGCCAAAACACGTACAGAGTCCAGCCGACATCGGCGCCCTGGTTCGAAGCACCCGCAAGGAGCAAAACCTGCGGCAGGA
CGAGCTTGCCGGCGTTGCCGGCGTCGGGCTTCGTTTTATCGTCGACCTCGAAGCCGGCAAGCCCACAGCGCAGATCGGCA
AAGTCCTGCAGGTTCTGCAAACGCTTGGCTGCTCCCTCGACATTCTGGCTCCCGGCGAGCGCAGGCAATGA

Structures

Toxin

• AlphaFold DB: W0IE92