Detailed information of TA system TA046013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4863489..4864111
• Replicon: chromosome
• Accession: NZ_CP007044
• Organism: Chania multitudinisentens RB-25

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: Z042_RS21650
• Protein ID: WP_024913899.1
• Coordinates: 4863908..4864111 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: W0LC89
• Locus tag: Z042_RS21645
• Protein ID: WP_024913900.1
• Coordinates: 4863489..4863857 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Z042_RS21630	4858766..4861912	+	3147	WP_024913903.1	multidrug efflux RND transporter permease subunit	-
Z042_RS21635	4862084..4862341	+	258	WP_024913902.1	type B 50S ribosomal protein L31	-
Z042_RS21640	4862354..4862494	+	141	WP_024913901.1	type B 50S ribosomal protein L36	-
Z042_RS21645	4863489..4863857	+	369	WP_024913900.1	Hha toxicity modulator TomB	Antitoxin
Z042_RS21650	4863908..4864111	+	204	WP_024913899.1	hemolysin expression modulator Hha	Toxin
Z042_RS21660	4864979..4865293	+	315	WP_024913898.1	MGMT family protein	-
Z042_RS21665	4865472..4866335	+	864	WP_024913897.1	acyl-CoA thioesterase II	-
Z042_RS21670	4866419..4867705	-	1287	WP_037407182.1	ammonium transporter AmtB	-
Z042_RS21675	4867743..4868081	-	339	WP_024913895.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8102.44 Da
• Isoelectric Point: 7.9816

>T46013 WP_024913899.1 NZ_CP007044:4863908-4864111 [Chania multitudinisentens RB-25]
MTKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELELFYSAADHRLAELTMNKLYDKIPTAVWKYVR

Nucleotide

• Length: 204 bp

>T46013 NZ_CP007044:4863908-4864111 [Chania multitudinisentens RB-25]
ATGACGAAAATTGACTATCTGATGCGTTTGCGTAAATGCACCACAATTGACACTCTGGAACGTGTTATAGAGAAAAATAA
GTATGAGCTCTCGAACGATGAGTTAGAGCTGTTCTATTCCGCAGCTGACCATCGCTTGGCTGAACTCACCATGAACAAAC
TGTACGATAAAATCCCAACTGCTGTTTGGAAATATGTAAGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14168.87 Da
• Isoelectric Point: 4.3849

>AT46013 WP_024913900.1 NZ_CP007044:4863489-4863857 [Chania multitudinisentens RB-25]
MDEYSPKRHDIVQLKFLCENLYDEGMATLGDSNHGWVNDPTSSVNLQLNELIEHIASFVMSFKIKYMGESDLSELVEEYL
DDTYTLFSNYGINDSDLRRWQKTRARLFGMFSGEGIYTTMKT

Nucleotide

• Length: 369 bp

>AT46013 NZ_CP007044:4863489-4863857 [Chania multitudinisentens RB-25]
ATGGATGAGTATTCGCCAAAAAGGCATGATATTGTACAACTCAAGTTTTTGTGTGAAAATTTGTACGACGAAGGCATGGC
CACTTTGGGTGACAGCAACCATGGCTGGGTAAATGACCCAACATCCTCCGTCAATCTGCAGCTTAACGAGTTGATAGAAC
ATATTGCTTCGTTTGTAATGAGTTTTAAAATTAAATACATGGGCGAAAGTGATTTGTCAGAGCTTGTCGAAGAGTATCTC
GATGATACCTATACATTGTTCAGCAATTACGGTATTAATGATTCCGACCTGCGTCGCTGGCAAAAAACCAGGGCGCGATT
ATTCGGGATGTTCTCAGGCGAAGGCATCTATACGACGATGAAAACTTAG

Structures

Antitoxin

• AlphaFold DB: W0LC89