Detailed information of TA system TA046004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3048101..3048654
• Replicon: chromosome
• Accession: NZ_CP007044
• Organism: Chania multitudinisentens RB-25

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: W0L4G7
• Locus tag: Z042_RS13315
• Protein ID: WP_024913593.1
• Coordinates: 3048340..3048654 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W0L8L2
• Locus tag: Z042_RS13310
• Protein ID: WP_024913594.1
• Coordinates: 3048101..3048337 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Z042_RS13285	3043416..3044018	+	603	WP_024913599.1	flavodoxin	-
Z042_RS13290	3044131..3044583	-	453	WP_024913598.1	helix-turn-helix transcriptional regulator	-
Z042_RS13295	3044777..3045967	+	1191	WP_202901349.1	MFS transporter	-
Z042_RS13300	3046146..3047369	+	1224	WP_024913596.1	hypothetical protein	-
Z042_RS13305	3047630..3047962	+	333	WP_024913595.1	hypothetical protein	-
Z042_RS13310	3048101..3048337	+	237	WP_024913594.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
Z042_RS13315	3048340..3048654	+	315	WP_024913593.1	CcdB family protein	Toxin
Z042_RS13320	3049194..3051356	-	2163	WP_024913592.1	extracellular solute-binding protein	-
Z042_RS13325	3051779..3052462	+	684	WP_024913591.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11472.42 Da
• Isoelectric Point: 8.8839

>T46004 WP_024913593.1 NZ_CP007044:3048340-3048654 [Chania multitudinisentens RB-25]
MQFTVYGNTGKSVVYPLLLNVTSDIIGQLNRRIVIPLLPIEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRSQVKAAIDFLIDGV

Nucleotide

• Length: 315 bp

>T46004 NZ_CP007044:3048340-3048654 [Chania multitudinisentens RB-25]
ATGCAATTCACTGTATACGGTAATACCGGGAAAAGCGTCGTTTACCCGCTGTTGCTCAATGTCACGAGCGATATTATTGG
ACAATTGAATCGTCGGATAGTGATCCCATTGCTCCCGATTGAAAAGTATCCGGCAGGCCGCCGACCGGATCGCCTTGTCC
CCGTCGTCAGGCTGACGGACGGCAAAGAATACGCCGTAATGACCCATGAGCTGGCAAGTATCCCTGTCCAGGCCCTTGGT
GCGGTGTTTTGTGATGCTTCGCAGTACCGTTCCCAGGTAAAGGCGGCAATAGATTTCCTTATTGACGGAGTTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9019.08 Da
• Isoelectric Point: 7.1660

>AT46004 WP_024913594.1 NZ_CP007044:3048101-3048337 [Chania multitudinisentens RB-25]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLTSALNTELRYHEAKKWQEENREALAALNRFHDEQGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT46004 NZ_CP007044:3048101-3048337 [Chania multitudinisentens RB-25]
ATGACCGCGAAACAACGCAACACGCAGAGCGTGACCATGACAGTAGAACGTGCCTTACTGGTAAGAGCGCGTGAAGCAGG
CATTAATCTGAGTGCTACCCTGACAAGCGCCCTGAATACAGAGCTTCGCTATCATGAAGCGAAAAAATGGCAAGAAGAAA
ACAGGGAGGCTCTCGCAGCACTAAATCGTTTTCATGATGAGCAGGGTTGTTTCAGCGATGAATATAGGACGTTTTAA

Structures

Toxin

• AlphaFold DB: W0L4G7

Antitoxin

• AlphaFold DB: W0L8L2