Detailed information of TA system TA046002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2104175..2104767
• Replicon: chromosome
• Accession: NZ_CP007044
• Organism: Chania multitudinisentens RB-25

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W0LAK4
• Locus tag: Z042_RS09330
• Protein ID: WP_024910049.1
• Coordinates: 2104393..2104767 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W0L674
• Locus tag: Z042_RS09325
• Protein ID: WP_024910048.1
• Coordinates: 2104175..2104396 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Z042_RS09310	2100936..2102034	+	1099	WP_154666930.1	peptide chain release factor 2	-
Z042_RS09315	2102044..2103561	+	1518	WP_024910047.1	lysine--tRNA ligase	-
Z042_RS09325	2104175..2104396	+	222	WP_024910048.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
Z042_RS09330	2104393..2104767	+	375	WP_024910049.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
Z042_RS25590	2105121..2105357	+	237	WP_024910050.1	Arm DNA-binding domain-containing protein	-
Z042_RS09340	2105449..2105796	-	348	WP_024910051.1	DUF1493 family protein	-
Z042_RS09345	2105787..2106236	-	450	WP_024910052.1	hypothetical protein	-
Z042_RS25595	2106714..2107075	+	362	Protein_1845	hypothetical protein	-
Z042_RS09350	2107080..2107496	+	417	WP_024910053.1	hypothetical protein	-
Z042_RS24930	2108064..2108249	+	186	WP_071882813.1	HNH endonuclease	-
Z042_RS09355	2108387..2109199	+	813	WP_024910054.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (7-111)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-55)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13923.81 Da
• Isoelectric Point: 5.5298

>T46002 WP_024910049.1 NZ_CP007044:2104393-2104767 [Chania multitudinisentens RB-25]
MKWVNASEIVIFHDRILQLYPGVAGMPDAGRAEAIIHRVHNFAHYEGVNDVFDLAAAYWVAIARGHIFNDDNKRTAFFTT
MAFLRRNGVLIRDEGNTLEELTVQAATGELTPIQLAQSLRELAE

Nucleotide

• Length: 375 bp

>T46002 NZ_CP007044:2104393-2104767 [Chania multitudinisentens RB-25]
ATGAAGTGGGTGAACGCCAGTGAAATAGTCATATTCCACGACAGGATTTTGCAGCTTTATCCCGGTGTGGCAGGTATGCC
AGATGCGGGCAGGGCAGAGGCCATTATTCATCGGGTTCACAATTTTGCTCACTATGAAGGTGTGAATGATGTGTTTGACT
TGGCGGCTGCCTATTGGGTTGCTATCGCGCGCGGGCATATCTTCAATGATGACAACAAGCGAACGGCATTCTTTACCACC
ATGGCGTTTCTAAGGCGTAACGGTGTGCTAATCCGCGATGAGGGTAACACGCTTGAAGAACTGACCGTCCAGGCCGCTAC
TGGTGAACTTACTCCGATACAATTAGCGCAAAGCTTACGCGAGCTTGCCGAATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8001.00 Da
• Isoelectric Point: 5.0190

>AT46002 WP_024910048.1 NZ_CP007044:2104175-2104396 [Chania multitudinisentens RB-25]
MLVYTSTQARANISAVLDAATSGEPVEITRRDGSAAIVISKAEFEAYQKAKLDAEFDSLMQRHGRTVEVLTDR

Nucleotide

• Length: 222 bp

>AT46002 NZ_CP007044:2104175-2104396 [Chania multitudinisentens RB-25]
ATGCTCGTTTACACGTCTACACAGGCGCGCGCCAATATTTCAGCAGTTCTTGACGCAGCCACCAGCGGGGAACCGGTAGA
AATTACTCGTCGTGATGGTTCTGCCGCCATAGTGATCAGCAAGGCCGAGTTTGAGGCATACCAAAAGGCAAAACTTGATG
CCGAATTCGACAGCCTTATGCAGCGCCACGGGCGGACGGTTGAGGTTTTGACTGATAGATGA

Structures

Toxin

• AlphaFold DB: W0LAK4

Antitoxin

• AlphaFold DB: W0L674