Detailed information of TA system TA046000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1327760..1328499
• Replicon: chromosome
• Accession: NZ_CP007044
• Organism: Chania multitudinisentens RB-25

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: W0L8C6
• Locus tag: Z042_RS05710
• Protein ID: WP_024914406.1
• Coordinates: 1327760..1328239 (-)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: W0LDE1
• Locus tag: Z042_RS05715
• Protein ID: WP_024914407.1
• Coordinates: 1328236..1328499 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Z042_RS05690	1323131..1323673	-	543	WP_024914403.1	type 1 fimbrial major subunit FimA	-
Z042_RS05695	1324881..1325333	+	453	WP_024914404.1	SgcJ/EcaC family oxidoreductase	-
Z042_RS05700	1325652..1326371	+	720	WP_004709363.1	two-component system response regulator OmpR	-
Z042_RS05705	1326368..1327738	+	1371	WP_024914405.1	two-component system sensor histidine kinase EnvZ	-
Z042_RS05710	1327760..1328239	-	480	WP_024914406.1	type II toxin-antitoxin system VapC family toxin	Toxin
Z042_RS05715	1328236..1328499	-	264	WP_024914407.1	Arc family DNA-binding protein	Antitoxin
Z042_RS05720	1328656..1330275	-	1620	WP_024914408.1	phosphoenolpyruvate carboxykinase (ATP)	-
Z042_RS05725	1330473..1331351	-	879	WP_024914409.1	Hsp33 family molecular chaperone HslO	-
Z042_RS05730	1331431..1331835	-	405	WP_024914410.1	ribosome-associated heat shock protein Hsp15	-
Z042_RS05735	1331825..1332511	-	687	WP_024914411.1	GMP/IMP nucleotidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (11-43)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 18254.94 Da
• Isoelectric Point: 5.6280

>T46000 WP_024914406.1 NZ_CP007044:c1328239-1327760 [Chania multitudinisentens RB-25]
MIILDTNVVAELLRPTPHYTVLKWLDEQDTYQFYLSAIVVAELYTGITCMPAGKRQLELRNRLDLMLQEEFPERILPFDT
DCAIQYAELMGANRRRGTLVSMADHQIAATCQQYGATLATRNTKDFIHSGITLIDPWQATTGRRLHEEAAEYYVMSRKP

Nucleotide

• Length: 480 bp

>T46000 NZ_CP007044:c1328239-1327760 [Chania multitudinisentens RB-25]
ATGATCATTCTCGATACCAATGTCGTTGCAGAGTTACTGCGCCCAACCCCTCATTACACGGTTTTAAAGTGGCTGGATGA
GCAGGATACTTATCAGTTTTATTTAAGTGCCATCGTGGTAGCAGAACTCTATACCGGCATCACCTGTATGCCCGCAGGGA
AAAGGCAGCTTGAACTGCGAAACCGTTTAGATCTGATGCTACAGGAAGAGTTTCCAGAAAGGATTTTACCTTTTGACACT
GATTGCGCCATACAGTACGCAGAACTAATGGGGGCCAATCGCCGTCGCGGTACTCTTGTCAGCATGGCAGACCATCAAAT
TGCCGCCACCTGCCAGCAATACGGCGCAACCTTGGCCACACGTAATACCAAGGATTTCATTCATAGCGGTATTACTCTTA
TCGACCCTTGGCAGGCCACCACTGGCCGACGCTTGCATGAAGAAGCCGCAGAATACTACGTGATGAGCAGAAAACCCTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9854.21 Da
• Isoelectric Point: 5.6343

>AT46000 WP_024914407.1 NZ_CP007044:c1328499-1328236 [Chania multitudinisentens RB-25]
MATITVRNLDDEVKELLRISAAKNGHSMEEEVRMILKQALTKKPPRYGLGTRLHQHFAEFGGVELEIPPRDATPPRIVTF
DNDDEQA

Nucleotide

• Length: 264 bp

>AT46000 NZ_CP007044:c1328499-1328236 [Chania multitudinisentens RB-25]
ATGGCGACTATCACCGTCAGGAATCTGGATGATGAAGTAAAAGAGCTGCTGCGTATCTCAGCGGCTAAAAATGGCCATTC
CATGGAGGAGGAAGTACGTATGATTTTGAAACAGGCGCTGACGAAAAAGCCACCGCGCTATGGGTTGGGAACCCGCCTGC
ATCAGCATTTTGCTGAATTCGGCGGTGTTGAACTGGAAATCCCACCACGTGACGCAACACCGCCACGCATTGTTACTTTT
GACAATGATGATGAACAAGCATGA

Structures

Toxin

• AlphaFold DB: W0L8C6

Antitoxin

• AlphaFold DB: W0LDE1