Detailed information of TA system TA045997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 426515..426977
• Replicon: chromosome
• Accession: NZ_CP007044
• Organism: Chania multitudinisentens RB-25

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: Z042_RS24705
• Protein ID: WP_071882780.1
• Coordinates: 426702..426977 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W0LED9
• Locus tag: Z042_RS01885
• Protein ID: WP_024910515.1
• Coordinates: 426515..426715 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Z042_RS01850	421792..423282	-	1491	WP_024910522.1	TIGR03752 family integrating conjugative element protein	-
Z042_RS01855	423272..424219	-	948	WP_045784775.1	TIGR03749 family integrating conjugative element protein	-
Z042_RS01860	424219..424872	-	654	WP_024910520.1	TIGR03746 family integrating conjugative element protein	-
Z042_RS01865	424865..425248	-	384	WP_024910519.1	TIGR03750 family conjugal transfer protein	-
Z042_RS01870	425261..425638	-	378	WP_024910518.1	TIGR03745 family integrating conjugative element membrane protein	-
Z042_RS01875	425667..425903	-	237	WP_024910517.1	TIGR03758 family integrating conjugative element protein	-
Z042_RS01880	425903..426214	-	312	WP_024910516.1	conjugal transfer protein	-
Z042_RS01885	426515..426715	+	201	WP_024910515.1	hypothetical protein	Antitoxin
Z042_RS24705	426702..426977	+	276	WP_071882780.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
Z042_RS01890	426999..427337	+	339	WP_024910514.1	hypothetical protein	-
Z042_RS01895	427384..428148	-	765	WP_024910513.1	TIGR03747 family integrating conjugative element membrane protein	-
Z042_RS01900	428157..428432	-	276	WP_024910512.1	hypothetical protein	-
Z042_RS01905	428445..430541	-	2097	WP_024910511.1	type IV conjugative transfer system coupling protein TraD	-
Z042_RS01910	430534..431127	-	594	WP_024910510.1	hypothetical protein	-
Z042_RS01915	431133..431681	-	549	WP_024910509.1	restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	391559..446895	55336

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10824.39 Da
• Isoelectric Point: 6.8475

>T45997 WP_071882780.1 NZ_CP007044:426702-426977 [Chania multitudinisentens RB-25]
VQWVKWKESALYDLAEIIDYIEQYNPAASSKLHHQIVHVTENLPQHPYLYRPGRVPGTREIVVHPNYLVIYHISELQIEI
LRVLHARQEYP

Nucleotide

• Length: 276 bp

>T45997 NZ_CP007044:426702-426977 [Chania multitudinisentens RB-25]
GTGCAATGGGTTAAATGGAAAGAATCAGCACTGTATGACTTGGCAGAGATCATAGATTATATTGAACAATACAACCCGGC
AGCGAGCAGCAAGTTACACCATCAGATAGTACACGTCACAGAGAACCTTCCTCAACATCCCTACCTTTATCGCCCTGGAA
GGGTTCCCGGTACACGGGAAATTGTCGTTCACCCTAACTATCTCGTTATTTACCATATTAGCGAGTTGCAAATCGAAATT
TTGCGAGTTCTACATGCTCGCCAAGAATATCCTTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7558.59 Da
• Isoelectric Point: 5.8234

>AT45997 WP_024910515.1 NZ_CP007044:426515-426715 [Chania multitudinisentens RB-25]
MKTKLSPIVSEFETQEQADSYDRWFRANVQAALDDPRPGIPHDEAMATLEKLLEEKRALRAKRAMG

Nucleotide

• Length: 201 bp

>AT45997 NZ_CP007044:426515-426715 [Chania multitudinisentens RB-25]
ATGAAAACCAAACTGTCCCCCATTGTGTCCGAATTTGAAACACAGGAGCAGGCAGACAGCTATGACCGCTGGTTTCGTGC
CAACGTGCAGGCAGCACTGGACGATCCACGCCCTGGTATTCCACATGATGAAGCAATGGCAACATTAGAGAAATTGCTGG
AAGAAAAACGGGCATTGAGGGCAAAACGTGCAATGGGTTAA

Structures

Antitoxin

• AlphaFold DB: W0LED9