45995

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 5254752..5256481 Replicon chromosome
Accession NZ_CP007039
Organism Pseudomonas cichorii JBC1

Toxin (Protein)


Gene name pmenT Uniprot ID A0A3M4WAB2
Locus tag BH81_RS22350 Protein ID WP_025261997.1
Coordinates 5254752..5255396 (+) Length 215 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A3M4WAC2
Locus tag BH81_RS22355 Protein ID WP_025261998.1
Coordinates 5255393..5256481 (+) Length 363 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BH81_RS22325 5250205..5250852 + 648 WP_025261992.1 DedA family protein -
BH81_RS22330 5250856..5251668 + 813 WP_025261993.1 zinc-dependent peptidase -
BH81_RS22335 5251773..5252300 + 528 WP_025261994.1 inorganic diphosphatase -
BH81_RS22340 5252645..5253376 + 732 WP_025261995.1 helix-turn-helix transcriptional regulator -
BH81_RS22345 5254174..5254755 + 582 WP_025261996.1 DUF4433 domain-containing protein -
BH81_RS22350 5254752..5255396 + 645 WP_025261997.1 DUF4433 domain-containing protein Toxin
BH81_RS22355 5255393..5256481 + 1089 WP_025261998.1 macro domain-containing protein Antitoxin
BH81_RS22360 5256624..5256929 - 306 WP_095067817.1 transcriptional regulator -
BH81_RS22365 5257244..5257483 - 240 WP_038400615.1 hypothetical protein -
BH81_RS27055 5257766..5258260 - 495 WP_176767542.1 hypothetical protein -
BH81_RS27060 5258390..5258695 - 306 Protein_4414 hypothetical protein -
BH81_RS27065 5258879..5259211 - 333 Protein_4415 Ig family protein -
BH81_RS27070 5259206..5259445 - 240 Protein_4416 hypothetical protein -
BH81_RS27075 5259602..5259982 - 381 Protein_4417 hypothetical protein -
BH81_RS26720 5260139..5260519 - 381 Protein_4418 putative Ig domain-containing protein -
BH81_RS26725 5260712..5261101 - 390 Protein_4419 putative Ig domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5254174..5268597 14423


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-213)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 215 a.a.        Molecular weight: 24844.35 Da        Isoelectric Point: 6.6571

>T45995 WP_025261997.1 NZ_CP007039:5254752-5255396 [Pseudomonas cichorii JBC1]
MNYTSLNPEKALIWRIVHRDNLPWILDNGLHCASSDVQAPQYVNIGNADLIDKRRSRDVPIAPMGVLADYVPFYFTPFSV
MMKNIHSGWSVQQRSNDEIVILVSSLHRVEQLGLPFVFTNAHAYPDWTNYYSDLAHLSEIDWPILQRRDFKRDPDDPRKM
ERYQAEALIHHHLPITGLLGIMCYTDAMKERIEQDVVARGLTLSVHARPGWYFQ

Download         Length: 645 bp

>T45995 NZ_CP007039:5254752-5255396 [Pseudomonas cichorii JBC1]
ATGAACTACACCAGTCTGAATCCAGAGAAGGCTCTGATTTGGCGTATCGTCCACCGCGATAACCTGCCTTGGATTCTGGA
CAACGGCCTGCATTGCGCCAGTTCGGATGTGCAAGCACCGCAATATGTAAATATTGGTAACGCTGACTTGATCGATAAAC
GCCGATCTCGCGATGTACCAATTGCTCCAATGGGTGTGTTGGCTGACTACGTGCCCTTCTACTTCACCCCCTTCTCTGTG
ATGATGAAAAATATTCATTCCGGGTGGAGCGTTCAGCAGCGCAGCAATGACGAGATTGTGATTCTGGTATCCAGCCTGCA
CCGCGTCGAACAGCTTGGCCTACCGTTCGTCTTCACAAACGCACATGCCTACCCGGATTGGACAAACTATTACAGCGATC
TGGCGCATCTCAGCGAGATCGACTGGCCTATCCTTCAGCGGCGCGACTTCAAACGCGATCCTGATGACCCTCGAAAGATG
GAGCGCTATCAGGCCGAAGCGCTGATTCACCACCACCTACCGATTACAGGACTCCTTGGCATCATGTGCTACACCGATGC
AATGAAAGAACGCATAGAGCAAGACGTGGTCGCTAGAGGCCTGACGTTGTCTGTTCATGCGCGTCCGGGATGGTATTTCC
AATGA

Antitoxin


Download         Length: 363 a.a.        Molecular weight: 40747.67 Da        Isoelectric Point: 6.3146

>AT45995 WP_025261998.1 NZ_CP007039:5255393-5256481 [Pseudomonas cichorii JBC1]
MIRFTQGNLLEAKTEALVNTVNTVGVMGKGIALMFKERFAENYRLYSAACKAGEVETGKVHVTAVNELEGPRWIVNFPTK
RHWRSPSQMQWITEGLHDLRRFLIENHVKSVAVPPLGAGNGGLKWPEVREQIVDALSDLDVDVLVFEPSSQYLNVAKRSG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIKNLPGTKNPLDLKFVAHKYGPYANRLEHLLDNLDGSYLH
CDKRISDADISDVIWFDQDRKAFLQTYLKTEAREYSQALELTAELIDGFESPFGMELLATVDWLLSQEEVSPTVPTVREA
LRHWDGGTGAAARKSKLFDDQAIDIALKRLTSSSFSSAVVNC

Download         Length: 1089 bp

>AT45995 NZ_CP007039:5255393-5256481 [Pseudomonas cichorii JBC1]
ATGATCAGATTCACTCAAGGCAACCTTCTGGAAGCCAAAACCGAAGCCCTCGTCAATACAGTGAATACCGTAGGCGTAAT
GGGCAAAGGTATCGCCCTGATGTTCAAAGAGCGCTTTGCGGAGAATTACCGCCTCTATTCGGCTGCCTGCAAGGCTGGCG
AGGTAGAAACAGGCAAAGTTCACGTGACCGCAGTCAATGAGCTGGAAGGCCCTCGCTGGATCGTAAACTTCCCAACCAAA
CGTCACTGGCGATCCCCTTCGCAGATGCAGTGGATAACCGAAGGCTTACACGATCTACGTCGCTTTTTGATTGAAAACCA
TGTTAAGTCCGTGGCGGTTCCTCCGCTAGGTGCAGGGAACGGTGGCTTGAAATGGCCAGAAGTTCGTGAGCAGATCGTTG
ATGCTTTGAGTGATCTGGACGTCGATGTGCTGGTATTCGAGCCATCCAGTCAGTATCTGAATGTCGCCAAACGTAGCGGA
GTGGAAAAGCTCACGCCTGCTCGCGCACTCATTGCCGAACTGGTTCGACGTTACTGGGTTTTAGGTATGGAATGCAGCCT
GCTTGAGATTCAGAAGCTGGCTTGGTTCTTAGAGCGAGCCATTAAAAATCTGCCGGGCACCAAAAACCCGCTGGATCTCA
AGTTCGTCGCCCATAAATACGGGCCGTATGCCAACCGATTGGAACACCTGCTCGACAATCTGGACGGCAGCTATCTGCAT
TGCGACAAACGCATCAGTGATGCAGATATCAGTGATGTGATCTGGTTTGACCAAGACCGTAAAGCTTTTCTGCAAACCTA
TCTCAAAACAGAAGCTAGGGAGTATTCCCAAGCTCTGGAACTGACCGCCGAACTGATCGATGGCTTTGAGTCACCGTTCG
GCATGGAGCTGTTGGCAACGGTTGATTGGTTGCTCAGTCAGGAAGAAGTTTCCCCAACCGTTCCTACCGTGCGCGAAGCG
TTGCGGCATTGGGATGGCGGAACTGGTGCTGCTGCTCGTAAAAGCAAGCTGTTCGATGATCAGGCGATTGATATTGCGCT
CAAACGGCTTACCTCCAGTAGCTTCAGCTCGGCAGTAGTTAACTGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3M4WAB2

Antitoxin

Source ID Structure
AlphaFold DB A0A3M4WAC2

References