Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 179649..180174 Replicon plasmid pG1663
Accession NZ_CP007038
Organism Shigella flexneri G1663

Toxin (Protein)


Gene name ccdB Uniprot ID Q326Z8
Locus tag SF2A_RS25855 Protein ID WP_001159860.1
Coordinates 179869..180174 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q7BEK0
Locus tag SF2A_RS25850 Protein ID WP_000813626.1
Coordinates 179649..179867 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SF2A_RS25795 (SF2A_25135) 175375..175560 + 186 Protein_209 ATP-binding protein -
SF2A_RS30540 (SF2A_25140) 175660..175869 - 210 Protein_210 peptidoglycan-binding protein -
SF2A_RS25805 (SF2A_25145) 175919..176170 - 252 WP_001381727.1 transporter -
SF2A_RS25810 (SF2A_25150) 176622..176906 + 285 WP_011114774.1 ribbon-helix-helix domain-containing protein -
SF2A_RS25815 (SF2A_25155) 176906..177181 + 276 WP_000421262.1 type II toxin-antitoxin system RelE/ParE family toxin -
SF2A_RS29335 178160..179113 + 954 Protein_215 IS66 family transposase -
SF2A_RS25850 (SF2A_25175) 179649..179867 + 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
SF2A_RS25855 (SF2A_25180) 179869..180174 + 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB Toxin
SF2A_RS30165 (SF2A_25185) 180198..180305 + 108 WP_023592908.1 transposase domain-containing protein -
SF2A_RS25860 (SF2A_25190) 180567..182204 + 1638 WP_000936806.1 T3SS effector E3 ubiquitin-protein ligase IpaH9.8 -
SF2A_RS25865 182412..182681 + 270 Protein_220 type II toxin-antitoxin system antitoxin YacA -
SF2A_RS31165 (SF2A_25200) 182681..182850 + 170 Protein_221 type II toxin-antitoxin system toxin YacB -
SF2A_RS25870 (SF2A_25205) 182933..183523 + 591 WP_000705601.1 type III secretion system effector protein kinase OspG -
SF2A_RS25880 183880..184146 + 267 Protein_223 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ipaH7.8 / ospC2 / ipaH7.8 / ipaH7.8 / ospD3/senA / ospC1 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA 1..222586 222586
- inside IScluster/Tn - ospI / ipaH9.8 / ospG 166120..186744 20624


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11736.59 Da        Isoelectric Point: 6.4674

>T45993 WP_001159860.1 NZ_CP007038:179869-180174 [Shigella flexneri G1663]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T45993 NZ_CP007038:179869-180174 [Shigella flexneri G1663]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8333.36 Da        Isoelectric Point: 6.2838

>AT45993 WP_000813626.1 NZ_CP007038:179649-179867 [Shigella flexneri G1663]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT45993 NZ_CP007038:179649-179867 [Shigella flexneri G1663]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTN3

Antitoxin

Source ID Structure
AlphaFold DB Q7BEK0

References