Detailed information of TA system TA045990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 45552..46327
• Replicon: plasmid pG1663
• Accession: NZ_CP007038
• Organism: Shigella flexneri G1663

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q7BEG9
• Locus tag: SF2A_RS24970
• Protein ID: WP_000405245.1
• Coordinates: 45552..46034 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q7BEG8
• Locus tag: SF2A_RS24975
• Protein ID: WP_010921625.1
• Coordinates: 46025..46327 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SF2A_RS26325	41967..43658	+	1692	Protein_58	E3 ubiquitin--protein ligase	-
SF2A_RS29085	43710..44918	+	1209	Protein_59	IS3 family transposase	-
SF2A_RS29090	44961..45484	-	524	Protein_60	transposase	-
SF2A_RS24970 (SF2A_24340)	45552..46034	-	483	WP_000405245.1	GNAT family N-acetyltransferase	Toxin
SF2A_RS24975 (SF2A_24345)	46025..46327	-	303	WP_010921625.1	DUF1778 domain-containing protein	Antitoxin
SF2A_RS24980 (SF2A_24350)	46599..46871	+	273	WP_000019158.1	hypothetical protein	-
SF2A_RS24985 (SF2A_24355)	46852..48059	+	1208	WP_094096874.1	IS91 family transposase	-
SF2A_RS30505	48231..48359	+	129	Protein_65	IS5/IS1182 family transposase	-
SF2A_RS24995 (SF2A_24360)	48508..49962	-	1455	WP_000701108.1	type 3 secretion system effector OspC2	-
SF2A_RS25005	50366..50985	+	620	Protein_67	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ospB / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ipaH7.8 / ospC2 / ipaH7.8 / ipaH7.8 / ospD3/senA / ospC1 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG / ipaH1.4 / ospE1 / nleE / icsP/sopA	1..222586	222586
	-	inside	IScluster/Tn	-	ospE2 / ipaH2.5 / ipaH7.8 / ospC2	32857..53344	20487

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17750.52 Da
• Isoelectric Point: 7.0382

>T45990 WP_000405245.1 NZ_CP007038:c46034-45552 [Shigella flexneri G1663]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T45990 NZ_CP007038:c46034-45552 [Shigella flexneri G1663]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTATTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11689.28 Da
• Isoelectric Point: 4.9167

>AT45990 WP_010921625.1 NZ_CP007038:c46327-46025 [Shigella flexneri G1663]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT45990 NZ_CP007038:c46327-46025 [Shigella flexneri G1663]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: Q7BEG9

Antitoxin

• AlphaFold DB: Q7BEG8