Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 2365506..2366133 Replicon chromosome
Accession NZ_CP007033
Organism Dehalobacter restrictus DSM 9455 strain PER-K23

Toxin (Protein)


Gene name pemK Uniprot ID K4L4V2
Locus tag DEHRE_RS11375 Protein ID WP_015043684.1
Coordinates 2365506..2365856 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1C2Y501
Locus tag DEHRE_RS11380 Protein ID WP_019225279.1
Coordinates 2365846..2366133 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DEHRE_RS11355 2360549..2361259 - 711 WP_019225283.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -
DEHRE_RS11360 2361332..2361757 - 426 WP_025206046.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -
DEHRE_RS11365 2361775..2362998 - 1224 WP_025206047.1 amidohydrolase family protein -
DEHRE_RS11370 2363196..2365334 - 2139 WP_019225280.1 RNA-binding transcriptional accessory protein -
DEHRE_RS11375 2365506..2365856 - 351 WP_015043684.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DEHRE_RS11380 2365846..2366133 - 288 WP_019225279.1 hypothetical protein Antitoxin
DEHRE_RS11385 2366293..2367093 - 801 WP_019225278.1 glutamine amidotransferase -
DEHRE_RS11390 2367090..2368517 - 1428 WP_025206048.1 Mur ligase family protein -
DEHRE_RS11395 2368762..2369311 + 550 Protein_2263 gamma carbonic anhydrase family protein -
DEHRE_RS11400 2369379..2369819 - 441 WP_025206049.1 MOSC domain-containing protein -
DEHRE_RS11405 2369836..2370333 - 498 WP_025206050.1 molybdopterin-guanine dinucleotide biosynthesis protein B -
DEHRE_RS11410 2370376..2370804 - 429 WP_025206051.1 sulfurase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-38)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12801.78 Da        Isoelectric Point: 5.2375

>T45968 WP_015043684.1 NZ_CP007033:c2365856-2365506 [Dehalobacter restrictus DSM 9455]
MSIKRGEIFYAELNPVVGSEQGGTRPVLVIQNDIGNQYSPTTIIAAITSQISKAKLPTHVEVKAKRSGLERDSVILTEQI
RTIDKSRLKEKVAVLDEEVMLRVDEAIEISLGLTEI

Download         Length: 351 bp

>T45968 NZ_CP007033:c2365856-2365506 [Dehalobacter restrictus DSM 9455]
ATGAGTATTAAACGCGGGGAGATTTTTTATGCGGAACTAAACCCCGTTGTAGGTTCTGAACAAGGCGGCACCAGGCCGGT
CTTGGTTATTCAGAATGATATTGGAAACCAGTATAGCCCGACAACAATTATCGCAGCAATTACATCCCAGATTTCAAAAG
CCAAACTGCCGACCCATGTGGAAGTCAAAGCTAAACGAAGCGGTTTGGAAAGAGATTCTGTTATTTTGACTGAACAAATC
AGAACGATCGATAAGAGCCGGTTGAAGGAAAAAGTCGCAGTGCTCGACGAGGAAGTAATGCTCAGGGTTGATGAAGCTAT
TGAAATAAGCCTTGGGCTGACGGAGATCTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10996.59 Da        Isoelectric Point: 4.3854

>AT45968 WP_019225279.1 NZ_CP007033:c2366133-2365846 [Dehalobacter restrictus DSM 9455]
MAETRRIVVCLPESIIEEVDEIVSSEKLNRSDFIKEAVYSVLIERRKAGIREQMRQGYVEMAQINLSMAVDLCQAEEEAT
MRYEGKLAWSVGYEY

Download         Length: 288 bp

>AT45968 NZ_CP007033:c2366133-2365846 [Dehalobacter restrictus DSM 9455]
TTGGCAGAGACTAGGCGTATTGTTGTATGTTTGCCGGAAAGCATTATTGAGGAAGTAGACGAAATCGTATCGTCAGAGAA
GTTAAACCGGAGTGATTTTATCAAAGAAGCTGTTTACAGTGTATTGATTGAACGGCGAAAAGCAGGAATACGAGAGCAGA
TGCGGCAGGGATATGTTGAAATGGCTCAAATCAACCTGTCGATGGCAGTAGATTTATGCCAGGCTGAAGAAGAGGCAACC
ATGCGTTATGAAGGGAAACTAGCTTGGAGTGTCGGGTATGAGTATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K4L4V2

Antitoxin

Source ID Structure
AlphaFold DB A0A1C2Y501

References