Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 830873..831498 Replicon chromosome
Accession NZ_CP007032
Organism Desulfitobacterium metallireducens DSM 15288

Toxin (Protein)


Gene name pemK Uniprot ID Q24Q87
Locus tag DESME_RS03980 Protein ID WP_005817423.1
Coordinates 831148..831498 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W0E652
Locus tag DESME_RS03975 Protein ID WP_025248646.1
Coordinates 830873..831148 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DESME_RS03955 826321..826839 - 519 WP_006715153.1 gamma carbonic anhydrase family protein -
DESME_RS03960 826875..828257 - 1383 WP_006715152.1 CZB domain-containing protein -
DESME_RS03965 828518..829912 + 1395 WP_006715151.1 DUF1727 domain-containing protein -
DESME_RS03970 829902..830666 + 765 WP_006715150.1 glutamine amidotransferase -
DESME_RS03975 830873..831148 + 276 WP_025248646.1 ribbon-helix-helix protein, CopG family Antitoxin
DESME_RS03980 831148..831498 + 351 WP_005817423.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DESME_RS03985 831604..832308 + 705 WP_006715148.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
DESME_RS03990 832395..833462 - 1068 WP_006715147.1 branched-chain amino acid aminotransferase -
DESME_RS03995 833588..835795 - 2208 WP_006715146.1 sigma-54-dependent Fis family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-39)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12824.86 Da        Isoelectric Point: 5.8944

>T45967 WP_005817423.1 NZ_CP007032:831148-831498 [Desulfitobacterium metallireducens DSM 15288]
MIIKRGEIYYAELNPVVGSEQGGTRPVLVIQNDIGNQFSPTTIIAAITSQIAKAKLPTHVEVRAKRSGLERDSVILTEQI
RTIDKSRLKEKVAVLDEEVMLRVDQAIEISLGLTDI

Download         Length: 351 bp

>T45967 NZ_CP007032:831148-831498 [Desulfitobacterium metallireducens DSM 15288]
ATGATTATAAAGCGCGGGGAAATTTACTACGCAGAACTTAACCCCGTCGTCGGCTCCGAGCAAGGCGGAACACGTCCCGT
CTTGGTGATTCAAAATGATATCGGAAATCAGTTTAGTCCGACGACTATCATTGCGGCGATCACTTCTCAGATTGCGAAAG
CTAAACTCCCCACTCATGTTGAAGTTCGTGCAAAACGGAGTGGACTCGAACGAGATTCAGTTATTTTAACCGAGCAAATT
CGGACCATTGATAAAAGTCGTTTAAAAGAGAAAGTAGCAGTGCTGGACGAAGAAGTTATGCTGAGAGTAGATCAAGCGAT
TGAAATTAGTTTGGGATTAACGGATATCTGA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10597.26 Da        Isoelectric Point: 6.4745

>AT45967 WP_025248646.1 NZ_CP007032:830873-831148 [Desulfitobacterium metallireducens DSM 15288]
MAESKRIMISLPESLLAEVDGIVTLEKRNRSEFIREAINSILHERRKKGIREQMIKGYKEMAQLNLAIAKELSSTEEEAS
QYFIEKHMECQ

Download         Length: 276 bp

>AT45967 NZ_CP007032:830873-831148 [Desulfitobacterium metallireducens DSM 15288]
TTGGCGGAAAGTAAGCGGATTATGATAAGTCTGCCAGAAAGTTTGCTTGCGGAAGTGGATGGGATAGTTACCTTAGAGAA
GAGAAATCGCAGCGAATTTATTCGAGAGGCTATTAATAGTATTCTTCACGAACGACGGAAGAAGGGAATCCGCGAGCAAA
TGATCAAAGGCTACAAAGAGATGGCTCAACTCAATTTGGCTATTGCTAAAGAATTGTCTTCTACAGAAGAGGAAGCTAGT
CAGTATTTTATTGAGAAACATATGGAGTGTCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M7T493

Antitoxin

Source ID Structure
AlphaFold DB W0E652

References