Detailed information of TA system TA045966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2149945..2150528
• Replicon: chromosome
• Accession: NZ_CP007031
• Organism: Marichromatium purpuratum 984

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: W0E4E4
• Locus tag: MARPU_RS09455
• Protein ID: WP_005225043.1
• Coordinates: 2150340..2150528 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: W0E3H4
• Locus tag: MARPU_RS09450
• Protein ID: WP_005225042.1
• Coordinates: 2149945..2150337 (-)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MARPU_RS16700	2146138..2146863	-	726	WP_005225037.1	phage antirepressor N-terminal domain-containing protein	-
MARPU_RS09435	2147409..2147588	-	180	WP_198015468.1	hypothetical protein	-
MARPU_RS16705	2148013..2148774	+	762	WP_025275252.1	helix-turn-helix transcriptional regulator	-
MARPU_RS17190	2148817..2149431	+	615	WP_005225040.1	BRCT domain-containing protein	-
MARPU_RS09445	2149468..2149926	+	459	WP_005225041.1	DUF4124 domain-containing protein	-
MARPU_RS09450	2149945..2150337	-	393	WP_005225042.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MARPU_RS09455	2150340..2150528	-	189	WP_005225043.1	type II toxin-antitoxin system HicA family toxin	Toxin
MARPU_RS17610	2150702..2150914	+	213	WP_005225044.1	hypothetical protein	-
MARPU_RS17615	2150911..2151075	+	165	WP_005225045.1	hypothetical protein	-
MARPU_RS17620	2151068..2151274	+	207	WP_005225046.1	hypothetical protein	-
MARPU_RS09460	2151271..2151594	+	324	WP_005225047.1	hypothetical protein	-
MARPU_RS09465	2151591..2152145	+	555	WP_005225048.1	hypothetical protein	-
MARPU_RS17195	2152179..2152325	+	147	Protein_1878	recombination-associated protein RdgC	-
MARPU_RS09470	2152355..2153161	+	807	WP_025275253.1	DNA adenine methylase	-
MARPU_RS17200	2153249..2153449	+	201	WP_198015469.1	DUF4224 domain-containing protein	-
MARPU_RS09475	2153530..2154429	+	900	WP_025275254.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2123063..2154429	31366

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-123)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7221.34 Da
• Isoelectric Point: 11.8399

>T45966 WP_005225043.1 NZ_CP007031:c2150528-2150340 [Marichromatium purpuratum 984]
MNSADVIRRLKAEGWVLAGGKGDHQKFKHPQRARHVVVPHPRKDLPVGTLRNIYRQAGWEWR

Nucleotide

• Length: 189 bp

>T45966 NZ_CP007031:c2150528-2150340 [Marichromatium purpuratum 984]
ATGAACAGCGCCGACGTGATTCGACGCCTGAAGGCTGAGGGCTGGGTTCTGGCCGGCGGCAAGGGCGATCATCAGAAGTT
CAAGCATCCGCAGCGGGCCCGGCATGTGGTGGTGCCGCACCCGCGCAAGGATCTTCCCGTCGGCACACTGCGCAACATCT
ACCGGCAAGCCGGTTGGGAATGGAGGTGA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 14436.48 Da
• Isoelectric Point: 5.1849

>AT45966 WP_005225042.1 NZ_CP007031:c2150337-2149945 [Marichromatium purpuratum 984]
MLYPAYVHLGDEQHAHGVTLPDFPGCFSAADTWEALPGAIQEAVELYFEGETLEIPAPTPLERLAHDPEYEGGVWLLVDI
DLARVRPKAMRLNISLPETLVRRIDAHAKARHLSRSAFLAQAARAAMEPR

Nucleotide

• Length: 393 bp

>AT45966 NZ_CP007031:c2150337-2149945 [Marichromatium purpuratum 984]
ATGCTCTACCCGGCGTATGTCCACTTGGGTGACGAACAGCACGCGCATGGCGTGACGCTCCCGGATTTCCCTGGCTGTTT
CTCGGCCGCCGATACCTGGGAGGCATTGCCGGGCGCGATTCAGGAAGCGGTCGAGCTCTACTTCGAGGGTGAGACGCTGG
AGATCCCGGCGCCGACGCCTCTGGAGAGACTGGCGCACGATCCTGAGTACGAGGGCGGGGTCTGGTTGTTGGTCGACATC
GATCTGGCGCGGGTGCGCCCCAAGGCGATGCGGCTCAATATCTCGCTGCCTGAGACCTTGGTGCGGCGCATCGATGCCCA
TGCCAAGGCTCGGCACTTGAGCCGCTCGGCATTCCTTGCTCAGGCGGCGCGGGCGGCAATGGAGCCGCGGTAG

Structures

Toxin

• AlphaFold DB: W0E4E4

Antitoxin

• AlphaFold DB: W0E3H4