Detailed information of TA system TA045961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 992406..992950
• Replicon: chromosome
• Accession: NZ_CP007030
• Organism: Thiomicrospira aerophila AL3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: THIAE_RS04735
• Protein ID: WP_025299319.1
• Coordinates: 992406..992681 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W0DRH8
• Locus tag: THIAE_RS04740
• Protein ID: WP_006460266.1
• Coordinates: 992681..992950 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THIAE_RS04715	987508..989031	+	1524	WP_006460261.1	lysine--tRNA ligase	-
THIAE_RS04720	989176..991416	+	2241	WP_157868723.1	RNA-binding transcriptional accessory protein	-
THIAE_RS04725	991479..991736	-	258	WP_025299318.1	hypothetical protein	-
THIAE_RS04730	992008..992331	+	324	WP_006460264.1	hypothetical protein	-
THIAE_RS04735	992406..992681	-	276	WP_025299319.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
THIAE_RS04740	992681..992950	-	270	WP_006460266.1	ribbon-helix-helix domain-containing protein	Antitoxin
THIAE_RS04745	993008..993439	-	432	WP_006460267.1	DUF4143 domain-containing protein	-
THIAE_RS04750	993490..993756	-	267	WP_025299320.1	hypothetical protein	-
THIAE_RS04755	994157..994870	-	714	WP_006460268.1	SDR family oxidoreductase	-
THIAE_RS04760	994867..995553	-	687	WP_006460269.1	HAD-IA family hydrolase	-
THIAE_RS04765	995556..996260	-	705	WP_041483050.1	bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-46)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10868.56 Da
• Isoelectric Point: 9.9482

>T45961 WP_025299319.1 NZ_CP007030:c992681-992406 [Thiomicrospira aerophila AL3]
IKLIWTSKATSDLVRLHAFLEPHNKQIAAKVTQSLAHAPNNLITHPRLGERLDEFIDREVRRILVGDYEMRYELQKDTIY
ILRIWHTKEHR

Nucleotide

• Length: 276 bp

>T45961 NZ_CP007030:c992681-992406 [Thiomicrospira aerophila AL3]
ATCAAACTGATCTGGACAAGCAAGGCAACGTCAGACCTTGTTCGACTTCATGCGTTTTTAGAACCACACAACAAACAGAT
AGCGGCTAAAGTCACACAATCACTCGCTCATGCACCCAACAATCTTATTACCCACCCAAGACTCGGTGAGCGACTTGATG
AATTTATCGACAGAGAGGTGCGCCGCATTTTAGTCGGTGACTATGAGATGCGTTACGAACTTCAAAAAGACACCATCTAT
ATATTACGCATCTGGCATACTAAAGAGCATCGTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10101.42 Da
• Isoelectric Point: 4.7628

>AT45961 WP_006460266.1 NZ_CP007030:c992950-992681 [Thiomicrospira aerophila AL3]
MIQTETRVLTAHIPLDMANQIDQLAGKLERSKSWIIKQALASWIEQESLRERLTREALADVDEGNFVSHDDIQAWAMSLN
SNQSKPTPI

Nucleotide

• Length: 270 bp

>AT45961 NZ_CP007030:c992950-992681 [Thiomicrospira aerophila AL3]
ATGATTCAAACAGAGACTCGTGTACTAACCGCACACATTCCACTTGATATGGCCAATCAAATAGACCAACTGGCGGGAAA
ACTTGAACGCTCAAAAAGCTGGATTATCAAACAAGCATTAGCTTCTTGGATTGAGCAAGAGTCCTTACGAGAACGCTTAA
CCCGTGAAGCACTTGCGGATGTTGACGAAGGCAACTTTGTTAGCCATGATGACATTCAAGCTTGGGCGATGAGTTTAAAT
AGTAATCAATCCAAGCCCACTCCAATATGA

Structures

Antitoxin

• AlphaFold DB: W0DRH8