Detailed information of TA system TA045960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 347267..347783
• Replicon: chromosome
• Accession: NZ_CP007030
• Organism: Thiomicrospira aerophila AL3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W0DUV7
• Locus tag: THIAE_RS01675
• Protein ID: WP_006459763.1
• Coordinates: 347267..347554 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W0DQC8
• Locus tag: THIAE_RS01680
• Protein ID: WP_006459764.1
• Coordinates: 347544..347783 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THIAE_RS01665	342538..343869	-	1332	WP_006459761.1	dihydroorotase	-
THIAE_RS01670	343951..347073	-	3123	WP_006459762.1	type I restriction endonuclease subunit R	-
THIAE_RS01675	347267..347554	-	288	WP_006459763.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
THIAE_RS01680	347544..347783	-	240	WP_006459764.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
THIAE_RS01685	347880..348167	-	288	WP_006459765.1	DUF2442 domain-containing protein	-
THIAE_RS01690	348335..349687	-	1353	WP_006459766.1	restriction endonuclease subunit S	-
THIAE_RS10785	349677..349832	-	156	WP_006459767.1	hypothetical protein	-
THIAE_RS01695	349829..351202	-	1374	WP_006459768.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-67)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11298.09 Da
• Isoelectric Point: 10.3023

>T45960 WP_006459763.1 NZ_CP007030:c347554-347267 [Thiomicrospira aerophila AL3]
MTYSLEFDQRALKEWHKLDATIQAQFKKKLAQVLEQPKIEANRLRDLENCYKIKLRQSGYRLVYQVQDARVVVFVIAIGK
RDKEKAYESAKHRQS

Nucleotide

• Length: 288 bp

>T45960 NZ_CP007030:c347554-347267 [Thiomicrospira aerophila AL3]
ATGACCTATAGTCTTGAATTTGATCAAAGAGCATTGAAAGAGTGGCATAAGCTTGACGCAACTATTCAAGCACAATTTAA
AAAGAAATTGGCACAAGTGCTTGAACAGCCAAAAATTGAAGCCAATCGACTGCGTGATTTGGAAAATTGTTACAAAATCA
AGCTACGTCAATCTGGTTATCGTCTGGTCTATCAAGTGCAGGATGCGCGTGTTGTGGTATTCGTTATTGCCATTGGCAAG
CGAGATAAAGAAAAAGCTTATGAGTCCGCGAAACATCGACAATCATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9137.46 Da
• Isoelectric Point: 4.7483

>AT45960 WP_006459764.1 NZ_CP007030:c347783-347544 [Thiomicrospira aerophila AL3]
MSTISIRVDDKLKSDAYQVLRELDITPSELLRQTLEYVAQHHKLPFKPVLLNEEDQALLQTVRERLQDPQPVAISLNDL

Nucleotide

• Length: 240 bp

>AT45960 NZ_CP007030:c347783-347544 [Thiomicrospira aerophila AL3]
ATGAGCACCATTAGTATTCGTGTCGATGACAAGTTAAAAAGTGATGCTTATCAAGTATTACGAGAGCTAGATATTACACC
ATCTGAATTGCTGCGCCAGACCTTAGAATATGTCGCACAACATCATAAACTGCCGTTTAAACCTGTATTACTCAACGAGG
AAGATCAGGCTTTACTGCAAACGGTGCGCGAAAGGTTACAAGATCCCCAACCCGTAGCGATTTCCCTAAATGACCTATAG

Structures

Toxin

• AlphaFold DB: W0DUV7

Antitoxin

• AlphaFold DB: W0DQC8