Detailed information of TA system TA045950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1127837..1128630
• Replicon: chromosome
• Accession: NZ_CP007029
• Organism: Thioalkalivibrio paradoxus ARh

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: W0DLB5
• Locus tag: THITH_RS05180
• Protein ID: WP_025367298.1
• Coordinates: 1128127..1128630 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: W0DKN1
• Locus tag: THITH_RS05175
• Protein ID: WP_006748765.1
• Coordinates: 1127837..1128115 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THITH_RS05145	1123604..1123795	-	192	WP_041483608.1	hypothetical protein	-
THITH_RS05150	1124065..1125000	+	936	WP_006748770.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
THITH_RS05155	1125000..1125659	+	660	WP_006748769.1	sulfoxide reductase heme-binding subunit YedZ	-
THITH_RS05160	1126043..1126363	+	321	WP_006748768.1	DUF2007 domain-containing protein	-
THITH_RS05165	1126529..1127083	+	555	WP_006748767.1	NUDIX hydrolase	-
THITH_RS05170	1127220..1127567	+	348	WP_006748766.1	hypothetical protein	-
THITH_RS05175	1127837..1128115	+	279	WP_006748765.1	DUF1778 domain-containing protein	Antitoxin
THITH_RS05180	1128127..1128630	+	504	WP_025367298.1	GNAT family N-acetyltransferase	Toxin
THITH_RS05185	1128921..1130237	+	1317	WP_006748763.1	nitrate regulatory protein	-
THITH_RS05190	1130701..1132035	+	1335	WP_006748762.1	ABC transporter substrate-binding protein	-
THITH_RS05195	1132051..1132980	+	930	WP_006748761.1	nitrate ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-88)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18412.24 Da
• Isoelectric Point: 7.7466

>T45950 WP_025367298.1 NZ_CP007029:1128127-1128630 [Thioalkalivibrio paradoxus ARh 1]
IASSYTPVRKLAATDHVDAFDCGQSALNQFLQRYALVNQKANSAQTYVCCQGDLVVGFYSLAVGSVDPEAAPSRVMKGLA
RHPVPVMILARLAVDREHQRLGLGQALLKDALLRTTQAADIAGIRCLLVHAKDEAARRWYESWEFEPSPTDPYHLFLMLK
DLKGMLG

Nucleotide

• Length: 504 bp

>T45950 NZ_CP007029:1128127-1128630 [Thioalkalivibrio paradoxus ARh 1]
ATCGCGTCGTCCTACACACCCGTTCGCAAGCTTGCTGCCACGGATCACGTCGATGCATTCGACTGCGGCCAGTCAGCGCT
GAACCAGTTCTTGCAGCGCTATGCACTCGTCAACCAGAAGGCCAACAGCGCGCAGACCTACGTCTGCTGCCAAGGTGACC
TGGTGGTCGGCTTCTACAGCCTCGCGGTTGGAAGCGTCGATCCGGAAGCCGCGCCGTCGAGGGTGATGAAGGGGTTGGCG
CGACACCCGGTGCCGGTCATGATCCTGGCCCGGCTCGCGGTGGACAGGGAACATCAGCGCCTGGGTCTGGGCCAGGCATT
GCTCAAGGATGCGCTGCTGCGCACGACGCAGGCTGCCGACATCGCCGGCATTCGCTGCCTGCTGGTGCATGCCAAGGACG
AAGCGGCGCGGCGCTGGTACGAGTCATGGGAATTCGAGCCCAGCCCGACCGATCCGTATCACCTGTTCCTGATGCTCAAG
GACCTCAAGGGCATGTTGGGCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10257.77 Da
• Isoelectric Point: 11.0836

>AT45950 WP_006748765.1 NZ_CP007029:1127837-1128115 [Thioalkalivibrio paradoxus ARh 1]
MSTRNLSKTERIDVRASAPVKLLLQEAARVSHKNVSEFLLDAGVTAAAQTLADRRQFVLSDAQWQAFQEALDRPVQSKPR
LSRLLREPGMLG

Nucleotide

• Length: 279 bp

>AT45950 NZ_CP007029:1127837-1128115 [Thioalkalivibrio paradoxus ARh 1]
ATGAGTACCCGGAATCTCTCCAAGACCGAACGCATCGACGTGCGCGCCAGCGCGCCCGTCAAGCTACTGCTGCAGGAAGC
AGCGCGCGTCAGCCACAAGAATGTCAGCGAGTTCCTGCTCGACGCGGGCGTGACGGCTGCCGCGCAGACCCTGGCGGATC
GTCGCCAGTTCGTGCTGAGTGATGCGCAGTGGCAGGCGTTCCAAGAGGCACTCGATCGTCCGGTGCAAAGCAAGCCGCGC
CTATCGAGGCTGCTACGTGAGCCTGGGATGCTGGGTTGA

Structures

Toxin

• AlphaFold DB: W0DLB5

Antitoxin

• AlphaFold DB: W0DKN1