Detailed information of TA system TA045947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 708833..709395
• Replicon: chromosome
• Accession: NZ_CP007029
• Organism: Thioalkalivibrio paradoxus ARh

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: W0DJN8
• Locus tag: THITH_RS03260
• Protein ID: WP_006745927.1
• Coordinates: 709027..709395 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: W0DGG7
• Locus tag: THITH_RS03255
• Protein ID: WP_006745928.1
• Coordinates: 708833..709030 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THITH_RS03235	704589..706454	-	1866	WP_006745932.1	GMC family oxidoreductase	-
THITH_RS03240	707230..707598	-	369	WP_006745931.1	cupin domain-containing protein	-
THITH_RS03245	707867..708151	+	285	WP_006745930.1	hypothetical protein	-
THITH_RS03250	708334..708759	+	426	WP_025367224.1	YchJ family protein	-
THITH_RS03255	708833..709030	+	198	WP_006745928.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
THITH_RS03260	709027..709395	+	369	WP_006745927.1	type II toxin-antitoxin system VapC family toxin	Toxin
THITH_RS03265	709979..711241	+	1263	WP_006745809.1	group II intron reverse transcriptase/maturase	-
THITH_RS03270	711348..711776	+	429	Protein_657	plasmid pRiA4b ORF-3 family protein	-
THITH_RS03275	711959..712144	+	186	WP_198019463.1	addiction module protein	-
THITH_RS03280	712223..712573	-	351	WP_006745924.1	DUF86 domain-containing protein	-
THITH_RS03285	712570..712860	-	291	WP_006745923.1	nucleotidyltransferase	-
THITH_RS03290	713032..713337	+	306	WP_006745922.1	nucleotidyltransferase domain-containing protein	-
THITH_RS03295	713412..713807	+	396	WP_006745921.1	hypothetical protein	-
THITH_RS03300	714041..714331	+	291	WP_025367226.1	nucleotidyltransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-46)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13447.50 Da
• Isoelectric Point: 4.8087

>T45947 WP_006745927.1 NZ_CP007029:709027-709395 [Thioalkalivibrio paradoxus ARh 1]
MILVDTSVWVDHLRAGDAVLVELLNDSQVLMHPFVLGELACGNLRNRGEVLRLLQHLPQAIVASDQEVLFFIERNALMGQ
GIGYVDAHLLAAVTLGGSTQLWTRDQRLRSVAEALKLAYGED

Nucleotide

• Length: 369 bp

>T45947 NZ_CP007029:709027-709395 [Thioalkalivibrio paradoxus ARh 1]
ATGATCCTGGTCGATACCTCGGTGTGGGTCGATCACCTGCGCGCCGGCGATGCCGTGCTGGTGGAGTTGCTGAACGACAG
TCAGGTGCTGATGCACCCTTTTGTGCTCGGTGAACTGGCCTGCGGCAACCTGCGCAACCGCGGTGAAGTGCTGCGGCTGC
TCCAGCATCTGCCGCAGGCGATCGTCGCCAGCGATCAGGAGGTACTGTTCTTCATCGAACGCAACGCGCTGATGGGCCAG
GGTATCGGCTATGTGGATGCGCACCTGCTGGCCGCCGTGACCCTGGGCGGCTCGACGCAACTGTGGACACGGGACCAGCG
GCTGCGGTCAGTGGCCGAGGCCTTGAAGCTTGCGTACGGCGAGGATTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7193.17 Da
• Isoelectric Point: 9.1096

>AT45947 WP_006745928.1 NZ_CP007029:708833-709030 [Thioalkalivibrio paradoxus ARh 1]
MRTTLNIDDQLLAEAQRVTGVSEKAALVREGLRALIERESARRLARLGGSEPQLEPVPRRQSDPA

Nucleotide

• Length: 198 bp

>AT45947 NZ_CP007029:708833-709030 [Thioalkalivibrio paradoxus ARh 1]
ATGCGCACGACGCTGAATATCGATGACCAGTTGCTGGCCGAGGCTCAGCGCGTTACCGGTGTGAGCGAAAAGGCCGCCCT
GGTACGGGAGGGGCTGCGCGCCTTGATCGAGCGCGAGAGCGCCCGGCGGCTGGCCCGGCTGGGCGGAAGTGAACCGCAAC
TGGAGCCCGTGCCCCGTCGGCAGTCCGATCCCGCATGA

Structures

Toxin

• AlphaFold DB: W0DJN8

Antitoxin

• AlphaFold DB: W0DGG7