Detailed information of TA system TA045946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 462236..462903
• Replicon: chromosome
• Accession: NZ_CP007029
• Organism: Thioalkalivibrio paradoxus ARh

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: THITH_RS02135
• Protein ID: WP_006746155.1
• Coordinates: 462236..462667 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: W0DJW3
• Locus tag: THITH_RS02140
• Protein ID: WP_006746154.1
• Coordinates: 462664..462903 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THITH_RS02085	458134..458514	+	381	WP_006746165.1	nucleotidyltransferase domain-containing protein	-
THITH_RS02090	458504..458938	+	435	WP_006746164.1	DUF86 domain-containing protein	-
THITH_RS02095	459036..459422	-	387	WP_006746163.1	type II toxin-antitoxin system VapC family toxin	-
THITH_RS02100	459422..459652	-	231	WP_006746162.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
THITH_RS02105	460022..460285	+	264	WP_006746161.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
THITH_RS02110	460694..461074	-	381	WP_006746160.1	helix-turn-helix transcriptional regulator	-
THITH_RS02115	461071..461286	-	216	WP_006746159.1	type II toxin-antitoxin system RelE/ParE family toxin	-
THITH_RS02120	461317..461517	-	201	WP_006746158.1	DUF2283 domain-containing protein	-
THITH_RS02125	461514..461774	-	261	WP_084222598.1	DUF4258 domain-containing protein	-
THITH_RS02130	461780..462226	-	447	Protein_430	hypothetical protein	-
THITH_RS02135	462236..462667	-	432	WP_006746155.1	type II toxin-antitoxin system VapC family toxin	Toxin
THITH_RS02140	462664..462903	-	240	WP_006746154.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
THITH_RS02145	463107..463500	+	394	Protein_433	nucleotidyltransferase domain-containing protein	-
THITH_RS02150	463782..464042	+	261	WP_006746152.1	prevent-host-death protein	-
THITH_RS02155	464048..464326	+	279	WP_006746151.1	type II toxin-antitoxin system YafQ family toxin	-
THITH_RS17390	464323..464523	-	201	WP_198019487.1	type II toxin-antitoxin system HicA family toxin	-
THITH_RS02160	464569..464799	-	231	WP_006746150.1	type II toxin-antitoxin system HicB family antitoxin	-
THITH_RS17405	465285..465536	+	252	WP_198019458.1	BrnT family toxin	-
THITH_RS02165	465553..465738	+	186	WP_198019488.1	hypothetical protein	-
THITH_RS02170	466103..467278	-	1176	WP_006746148.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	461317..474933	13616

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-127)

Antitoxin

• SpoVT-AbrB domain (10-49)

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15635.95 Da
• Isoelectric Point: 7.2807

>T45946 WP_006746155.1 NZ_CP007029:c462667-462236 [Thioalkalivibrio paradoxus ARh 1]
VTDTPLFLLDTNILSDLVRHPQGIVKRGIERVGEHAVCTSIIAAAGLRFGAAKRGSVALTEQVEKVLGAMRVEPLDLPSD
REYGRLRLQLEQTGQPIGPNDLLIAAHALALGATVVTDNQREFQRVPGLRVENWLQTNRPAEK

Nucleotide

• Length: 432 bp

>T45946 NZ_CP007029:c462667-462236 [Thioalkalivibrio paradoxus ARh 1]
GTGACGGACACACCGCTTTTCCTACTGGATACCAACATCCTCTCCGATCTGGTGCGCCACCCTCAGGGAATCGTCAAACG
CGGGATCGAGAGGGTCGGTGAGCACGCGGTCTGCACCTCCATCATCGCTGCTGCCGGACTACGGTTCGGTGCAGCGAAGC
GGGGATCCGTAGCGCTGACGGAGCAGGTGGAGAAGGTCCTTGGTGCCATGCGCGTAGAGCCGCTCGACCTACCCTCAGAT
CGCGAATACGGGCGGCTCCGTCTGCAACTCGAGCAGACCGGACAGCCCATCGGCCCGAATGACCTCCTGATCGCGGCGCA
CGCCTTGGCACTCGGCGCCACGGTCGTCACCGACAACCAGCGAGAATTCCAGCGCGTGCCCGGACTGCGGGTGGAGAACT
GGCTCCAGACGAATCGGCCAGCTGAGAAGTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9143.36 Da
• Isoelectric Point: 4.8668

>AT45946 WP_006746154.1 NZ_CP007029:c462903-462664 [Thioalkalivibrio paradoxus ARh 1]
METTRHVRLFRNGRNQAVRIPREFELDTEEAVIYREDDRLVIEPVRRKGLLATLATLHDLEEAFPNIDSGLGALDDVRL

Nucleotide

• Length: 240 bp

>AT45946 NZ_CP007029:c462903-462664 [Thioalkalivibrio paradoxus ARh 1]
ATGGAAACCACCCGTCATGTCCGACTCTTCCGCAACGGCCGTAACCAAGCGGTCCGGATTCCGCGGGAGTTCGAACTGGA
CACAGAAGAAGCCGTCATCTATCGGGAAGACGACCGCCTCGTGATCGAGCCCGTCCGGCGCAAGGGACTCCTTGCCACAC
TGGCCACACTGCACGATCTCGAAGAAGCGTTCCCCAATATTGATTCCGGGCTCGGCGCGCTGGACGACGTACGCCTGTGA

Structures

Antitoxin

• AlphaFold DB: W0DJW3