Detailed information of TA system TA045940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 72116..72651
• Replicon: chromosome
• Accession: NZ_CP007029
• Organism: Thioalkalivibrio paradoxus ARh

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W0DJ04
• Locus tag: THITH_RS00375
• Protein ID: WP_006746512.1
• Coordinates: 72328..72651 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: THITH_RS17240
• Protein ID: WP_198019482.1
• Coordinates: 72116..72331 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THITH_RS17005	67373..69361	-	1989	WP_006746517.1	hypothetical protein	-
THITH_RS00360	69615..70049	-	435	WP_006746516.1	pilin	-
THITH_RS00365	70779..71354	+	576	WP_084222694.1	Uma2 family endonuclease	-
THITH_RS00370	71429..72013	+	585	WP_006746514.1	Uma2 family endonuclease	-
THITH_RS17240	72116..72331	+	216	WP_198019482.1	antitoxin MazE family protein	Antitoxin
THITH_RS00375	72328..72651	+	324	WP_006746512.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
THITH_RS00380	72892..74610	-	1719	WP_006746511.1	NADH-ubiquinone oxidoreductase	-
THITH_RS00385	75003..76010	+	1008	WP_006746510.1	NAD-dependent epimerase	-
THITH_RS00390	76381..76869	+	489	WP_006746509.1	transcriptional activator RfaH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• Antitoxin MazE-like (4-69)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11419.35 Da
• Isoelectric Point: 10.2913

>T45940 WP_006746512.1 NZ_CP007029:72328-72651 [Thioalkalivibrio paradoxus ARh 1]
MKRGDLVTVAMSGDFGKPRPALVIQSDHFDATATVTVLLVSSTLVDAPLIRLAVEPSAENGLRKPSQVMIDKAMTVRRDR
VGAPIGRLEADTMVGVNRSLALFLGFA

Nucleotide

• Length: 324 bp

>T45940 NZ_CP007029:72328-72651 [Thioalkalivibrio paradoxus ARh 1]
ATGAAGCGCGGAGATCTGGTCACGGTGGCGATGTCGGGTGATTTCGGTAAGCCCCGGCCGGCGCTGGTGATTCAGTCCGA
CCACTTCGACGCTACGGCGACGGTGACGGTACTTCTGGTTTCCTCGACGCTCGTCGACGCTCCGCTGATCCGGCTGGCCG
TCGAGCCGAGCGCGGAGAATGGCCTGCGCAAGCCATCGCAGGTGATGATCGACAAGGCGATGACGGTGCGGCGGGATCGG
GTGGGCGCACCCATCGGTCGACTGGAAGCGGACACCATGGTTGGGGTGAACCGTAGCCTCGCGCTGTTTCTCGGGTTTGC
GTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8087.09 Da
• Isoelectric Point: 4.5105

>AT45940 WP_198019482.1 NZ_CP007029:72116-72331 [Thioalkalivibrio paradoxus ARh 1]
MSVNERVQKRRALLRASGLRPLQIWVPDTRQPGFVEECRRQSMVVARTDLDDPDVDTFMEGAPADADDWTA

Nucleotide

• Length: 216 bp

>AT45940 NZ_CP007029:72116-72331 [Thioalkalivibrio paradoxus ARh 1]
ATGTCAGTGAATGAACGTGTACAAAAGCGGCGCGCCCTGCTGCGAGCGTCCGGTTTACGGCCTCTGCAGATTTGGGTGCC
GGATACGCGGCAGCCGGGCTTCGTGGAGGAATGCCGGCGTCAATCGATGGTCGTCGCCCGGACGGATCTCGACGATCCCG
ATGTGGACACCTTCATGGAGGGTGCCCCGGCGGATGCCGACGACTGGACCGCATGA

Structures

Toxin

• AlphaFold DB: W0DJ04