Detailed information of TA system TA045939

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-PrlF
• Location: 60668..61232
• Replicon: chromosome
• Accession: NZ_CP007029
• Organism: Thioalkalivibrio paradoxus ARh

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: L0DQK6
• Locus tag: THITH_RS00330
• Protein ID: WP_006746522.1
• Coordinates: 60888..61232 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: L0DRW8
• Locus tag: THITH_RS00325
• Protein ID: WP_006746523.1
• Coordinates: 60668..60898 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THITH_RS00280	55762..55992	+	231	WP_006746532.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
THITH_RS00285	55994..56398	+	405	WP_006746531.1	DUF4411 family protein	-
THITH_RS00290	56458..56946	-	489	WP_006746530.1	DUF3368 domain-containing protein	-
THITH_RS00295	56928..57272	-	345	WP_006746529.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
THITH_RS00300	57579..58485	-	907	Protein_62	hypothetical protein	-
THITH_RS00305	58902..59168	+	267	WP_006746527.1	type II toxin-antitoxin system CcdA family antitoxin	-
THITH_RS00310	59168..59482	+	315	WP_006746526.1	CcdB family protein	-
THITH_RS00315	59549..59944	-	396	WP_006746525.1	type II toxin-antitoxin system VapC family toxin	-
THITH_RS00320	59949..60188	-	240	WP_006746524.1	hypothetical protein	-
THITH_RS00325	60668..60898	+	231	WP_006746523.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
THITH_RS00330	60888..61232	+	345	WP_006746522.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
THITH_RS00335	61338..62186	-	849	WP_025367142.1	transposase	-
THITH_RS00340	62504..63727	-	1224	WP_006746520.1	glycosyltransferase family 4 protein	-
THITH_RS00345	63717..64607	-	891	WP_006746519.1	glycosyltransferase family 2 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-110)

Antitoxin

• SpoVT-AbrB domain (5-31)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12168.08 Da
• Isoelectric Point: 10.7923

>T45939 WP_006746522.1 NZ_CP007029:60888-61232 [Thioalkalivibrio paradoxus ARh 1]
VTYDRFTVVRVPFPFTDRSAVKNRPALVLSDAAAFSATAGHSVMAMITSKGNAPWPLDCSISELDAAGLPAPSVVRFKLF
TLDHRLIRSTLGQLAEADRATVRSALKRLLGGAA

Nucleotide

• Length: 345 bp

>T45939 NZ_CP007029:60888-61232 [Thioalkalivibrio paradoxus ARh 1]
GTGACCTATGACCGCTTCACGGTCGTGCGTGTACCGTTTCCGTTCACCGACCGCAGCGCTGTCAAGAACCGCCCGGCCCT
GGTGCTTTCGGATGCCGCGGCCTTTAGCGCGACCGCTGGGCACTCGGTGATGGCGATGATTACTTCGAAAGGCAACGCAC
CCTGGCCACTCGATTGCTCAATCAGCGAACTTGACGCCGCCGGCCTGCCGGCGCCGTCCGTGGTGCGCTTCAAGCTGTTT
ACCCTCGATCACCGCCTGATTCGGAGCACCCTGGGCCAACTCGCTGAGGCCGACCGTGCAACCGTGCGCAGCGCATTGAA
GCGCTTGCTGGGCGGCGCCGCGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8350.46 Da
• Isoelectric Point: 4.9890

>AT45939 WP_006746523.1 NZ_CP007029:60668-60898 [Thioalkalivibrio paradoxus ARh 1]
MPAIARITAKGQTTIPKQVRAALHVAPGDLIAWEIHPDGTASVRRVQPLDVDYLRALEATLSEWSSPADEEAYRDL

Nucleotide

• Length: 231 bp

>AT45939 NZ_CP007029:60668-60898 [Thioalkalivibrio paradoxus ARh 1]
ATGCCCGCAATCGCGAGAATTACCGCCAAAGGCCAGACGACCATTCCGAAGCAGGTGCGCGCTGCCTTGCATGTCGCACC
CGGCGACCTCATCGCATGGGAGATCCATCCTGATGGGACTGCGTCGGTGCGGCGCGTGCAGCCTCTGGACGTGGACTACC
TGCGCGCCCTCGAAGCTACCCTGTCCGAGTGGTCCAGCCCGGCCGATGAGGAAGCTTACCGTGACCTATGA

Structures

Toxin

• AlphaFold DB: L0DQK6

Antitoxin

• AlphaFold DB: L0DRW8