Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3182526..3183213 Replicon chromosome
Accession NZ_CP007027
Organism Mycobacterium tuberculosis H37RvSiena

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag Y980_RS15180 Protein ID WP_003414624.1
Coordinates 3182770..3183213 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag Y980_RS15175 Protein ID WP_003414620.1
Coordinates 3182526..3182783 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y980_RS15155 3177846..3178700 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
Y980_RS15160 3178756..3179919 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
Y980_RS15165 3179936..3181150 - 1215 WP_003899518.1 zinc metalloprotease Rip -
Y980_RS15170 3181158..3182399 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
Y980_RS15175 3182526..3182783 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
Y980_RS15180 3182770..3183213 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
Y980_RS15185 3183293..3183955 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
Y980_RS15190 3184051..3184242 + 192 WP_003414632.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
Y980_RS15195 3184574..3186322 + 1749 WP_178121166.1 cytochrome c biogenesis protein DipZ -
Y980_RS15200 3186418..3186999 + 582 WP_003414644.1 fasciclin domain-containing protein -
Y980_RS15205 3187099..3187365 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T45933 WP_003414624.1 NZ_CP007027:3182770-3183213 [Mycobacterium tuberculosis H37RvSiena]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T45933 NZ_CP007027:3182770-3183213 [Mycobacterium tuberculosis H37RvSiena]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT45933 WP_003414620.1 NZ_CP007027:3182526-3182783 [Mycobacterium tuberculosis H37RvSiena]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT45933 NZ_CP007027:3182526-3182783 [Mycobacterium tuberculosis H37RvSiena]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References